LOCUS       sequence-398078-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus D68 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EV-D68 2A protease; no tag, low expression levels
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctcct cacatcatca ccaccaccac atggcgagca tgagtgacag tgaggtaaac
       61 caagaggcca agcccgaggt caagccggaa gtaaaacccg agacacacat caacttaaaa
      121 gtatctgacg gttcttctga gatattcttc aagattaaga agacgactcc actgcggaga
      181 ttaatggaag cattcgcaaa acggcaagga aaggaaatgg actctcttcg cttcctctac
      241 gacggcattc gtattcaagc cgatcaaacc cccgaggacc ttgacatgga agataacgac
      301 atcatcgagg cgcaccggga acaaatcggc ggtggccctg gattcggtgg cgtgttcgtt
      361 gggagtttca aaataataaa ttaccacctt gcaaccatag aggaacgcca gtccgcgatt
      421 tatgtcgatt ggcaatcgga cgtactggtg acccctatag ctgcgcatgg gcgacatcag
      481 atagctagat gtaaatgcaa taccggcgta tattattgtc gccaccggga ccgttcgtat
      541 cccatctgtt tcgaagggcc agggattcaa tggatcgagc aaaatgaata ctaccccgcg
      601 cggtatcaaa ctaacgtctt attggcggcc ggaccggcag aagctggcga ctgtggtgga
      661 ttgctcgtgt gccctcatgg ggtgataggt ttgttgactg caggaggagg tggcatagtt
      721 gcgttcacgg acataagaaa cttgctctgg ttagacactg acgtgatgga acagtaatga
//
