LOCUS       sequence-398082-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus A71 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EV-A71 2A protease; 6xHis-tag, self cleaving
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             547..564
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgaattata aaggaaatga tatcaagtgt acatcaacat cccgtgataa aataacgacc
       61 ttgggaaagt tcgggcaaca atcgggtgcc atatatgtgg gtaactatcg tgtggttaat
      121 cgccacctcg ctactcacaa tgattgggcc aacttagtgt gggaggacag ctctcgcgat
      181 ctccttgtat catcgaccac tgcgcagggt tgcgacacca ttgcgcgctg tgattgccag
      241 acaggcgtgt attattgctc gtctcgtcgt aaacattatc cggtttcttt ttctaagccg
      301 tcactgatct ttgttgaagc gtcagagtac tatccagccc gttatcagtc ccatctgatg
      361 ctggctgtgg gtcacagcga acccggggat tgtggcggta tactgcgctg tcagcacggt
      421 gtagttggca ttgtcagtac aggaggtaat gggttagtcg gatttgccga tgtgcgggat
      481 ttactgtggt tggacgagga ggctatggaa cagggcgtat ctgattatat caaaggactg
      541 ggcgaccacc atcatcacca ccattaatga
//
