LOCUS       sequence-398084-        1638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus A71 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1638)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EV-A71 2A protease; 6xHis-MBP-TEV tag, self cl...
FEATURES             Location/Qualifiers
     source          1..1638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1159..1179
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgaagatcc accatcatca tcatcacgaa gagggtaagc tggtgatctg gatcaatggg
       61 gataaggggt acaacggcct ggcagaggtc ggtaagaagt ttgagaagga cacagggatt
      121 aaggtgactg ttgagcaccc tgataagctg gaagaaaagt tcccgcaggt tgcagccacc
      181 ggagacggcc ctgacatcat tttctgggca cacgaccgct tcggtgggta cgcgcaatct
      241 ggactgttgg ctgaaattac gcccgacaag gcattccaag acaaattata ccctttcacg
      301 tgggatgctg tccggtataa cgggaagctg atagcgtatc cgattgcggt agaggcgttg
      361 tcattaatct ataacaagga tttattaccc aatccgccca aaacttggga agagatccca
      421 gcgttggaca aggagttgaa ggcaaagggt aaaagcgccc tcatgttcaa ccttcaagaa
      481 ccgtatttta cgtggccatt aatcgccgct gacggtgggt acgcattcaa gtacgagaac
      541 ggaaagtacg atatcaagga tgttggggtt gacaacgctg gggccaaagc gggcttaacg
      601 ttcctcgttg acctcataaa gaacaagcac atgaacgcgg atacggacta ctcgatagct
      661 gaagctgctt ttaacaaagg cgagactgct atgaccatta acggaccttg ggcgtggtcc
      721 aacattgaca catcaaaggt gaactacggg gttacagtac ttccgacctt taaagggcaa
      781 cctagcaagc cattcgtggg agtcctgtca gccggaatta atgcggcgtc accgaacaag
      841 gagttagcga aggagtttct tgagaactat ttacttaccg acgagggctt ggaagccgtt
      901 aacaaggaca agccacttgg ggcggtagca ttaaaatcgt acgaagaaga attggcgaag
      961 gacccgcgga tcgccgcgac gatggaaaat gcccagaagg gcgagattat gccgaacatt
     1021 ccccaaatgt ctgcattctg gtatgcagtt cgcacggcag tgataaacgc agccagcggt
     1081 cgtcaaacgg tggacgaggc tcttaaggat gcccaaacga actccataac atcgctgtac
     1141 aagaaggctg gatctggtga gaacctgtac ttccaatcca tgggaaagtt cgggcaacaa
     1201 tcgggtgcca tatatgtggg taactatcgt gtggttaatc gccacctcgc tactcacaat
     1261 gattgggcca acttagtgtg ggaggacagc tctcgcgatc tccttgtatc atcgaccact
     1321 gcgcagggtt gcgacaccat tgcgcgctgt gattgccaga caggcgtgta ttattgctcg
     1381 tctcgtcgta aacattatcc ggtttctttt tctaagccgt cactgatctt tgttgaagcg
     1441 tcagagtact atccagcccg ttatcagtcc catctgatgc tggctgtggg tcacagcgaa
     1501 cccggggatt gtggcggtat actgcgctgt cagcacggtg tagttggcat tgtcagtaca
     1561 ggaggtaatg ggttagtcgg atttgccgat gtgcgggatt tactgtggtt ggacgaggag
     1621 gctatggaac agtaatga
//
