LOCUS       sequence-398085-        1176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expresison of Enterovirus A71 2A protease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1176)
  TITLE     The SARS-Cov-2 main protease (Mpro) is responsible for viral 
            polyprotein processing and therefore a therapeutic target.
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Enterovirus A71 2A protease
FEATURES             Location/Qualifiers
     source          1..1176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..681
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             697..717
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc accaccatca ctcatcgatg tctcccattt tgggttattg gaagataaag
       61 gggttagtac aaccgaccag attactgtta gagtaccttg aagagaagta tgaagagcat
      121 ttatatgagc gcgacgaagg cgataagtgg cgcaacaaga aatttgagtt aggcctcgag
      181 tttccgaacc ttccttatta tatcgacggg gacgtaaagt tgacgcaatc tatggcaata
      241 attcggtata tagcagacaa gcataacatg ttgggtggct gccccaaaga gcgtgccgag
      301 atctccatgt tagaaggagc cgtcctggac attcgctatg gtgtgtcgcg cattgcctac
      361 tcaaaagatt tcgagacttt aaaagttgac tttctgagca agctgccgga gatgcttaag
      421 atgttcgaag atcggctgtg tcacaagacg tacctcaacg gtgaccacgt tacccaccca
      481 gactttatgc tttacgatgc gctggacgtt gtgctttaca tggacccgat gtgtctggac
      541 gcgtttccga agttggtatg tttcaagaag cggattgagg ctattcccca aatagacaaa
      601 taccttaaga gtagtaagta catcgcgtgg ccacttcaag gttggcaagc taccttcggc
      661 ggcggcgacc atcccccaaa gtcgggcggc ggcagcgaga acctgtactt ccaatccatg
      721 ggaaagttcg ggcaacaatc gggtgccata tatgtgggta actatcgtgt ggttaatcgc
      781 cacctcgcta ctcacaatga ttgggccaac ttagtgtggg aggacagctc tcgcgatctc
      841 cttgtatcat cgaccactgc gcagggttgc gacaccattg cgcgctgtga ttgccagaca
      901 ggcgtgtatt attgctcgtc tcgtcgtaaa cattatccgg tttctttttc taagccgtca
      961 ctgatctttg ttgaagcgtc agagtactat ccagcccgtt atcagtccca tctgatgctg
     1021 gctgtgggtc acagcgaacc cggggattgt ggcggtatac tgcgctgtca gcacggtgta
     1081 gttggcattg tcagtacagg aggtaatggg ttagtcggat ttgccgatgt gcgggattta
     1141 ctgtggttgg acgaggaggc tatggaacag taatga
//
