LOCUS       sequence-398097-       12021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human PTPN2 transcript variant 
            1 under the spleen focus-forming virus (SFFV) promoter and Emerald 
            under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12021)
  TITLE     PTPN2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLTR-SFFV-PTPN2 transcript variant
            1-WPRE-UbC-...
FEATURES             Location/Qualifiers
     source          1..12021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3773..3793)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4191..4202)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5241..5260
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5434..5443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5440..5442,5443..5445,5446..6159)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5485..5506)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5746..5765)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6093..6114
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6254..6487
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6512..6530)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6513..6530)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6820..6839)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7053..7072)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7172..7194
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7232..7250)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7318..7422
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7423..7491,7492..8283)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7641..7660)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8454..9042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8943..8962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9196..9213
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9283..9303)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9288..9617
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9468..9603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10751..10816
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10946..10966
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11391..11525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11428..11447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11482..11501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11693..12021,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccgcca ccatgcccac caccatcgag cgggagttcg aagagttgga tactcagcgt
     2461 cgctggcagc cgctgtactt ggaaattcga aatgagtccc atgactatcc tcatagagtg
     2521 gccaagtttc cagaaaacag aaatcgaaac agatacagag atgtaagccc atatgatcac
     2581 agtcgtgtta aactgcaaaa tgctgagaat gattatatta atgccagttt agttgacata
     2641 gaagaggcac aaaggagtta catcttaaca cagggtccac ttcctaacac atgctgccat
     2701 ttctggctta tggtttggca gcagaagacc aaagcagttg tcatgctgaa ccgcattgtg
     2761 gagaaagaat cggttaaatg tgcacagtac tggccaacag atgaccaaga gatgctgttt
     2821 aaagaaacag gattcagtgt gaagctcttg tcagaagatg tgaagtcgta ttatacagta
     2881 catctactac aattagaaaa tatcaatagt ggtgaaacca gaacaatatc tcactttcat
     2941 tatactacct ggccagattt tggagtccct gaatcaccag cttcatttct caatttcttg
     3001 tttaaagtga gagaatctgg ctccttgaac cctgaccatg ggcctgcggt gatccactgt
     3061 agtgcaggca ttgggcgctc tggcaccttc tctctggtag acacttgtct tgttttgatg
     3121 gaaaaaggag atgatattaa cataaaacaa gtgttactga acatgagaaa ataccgaatg
     3181 ggtcttattc agaccccaga tcaactgaga ttctcataca tggctataat agaaggagca
     3241 aaatgtataa agggagattc tagtatacag aaacgatgga aagaactttc taaggaagac
     3301 ttatctcctg cctttgatca ttcaccaaac aaaataatga ctgaaaaata caatgggaac
     3361 agaataggtc tagaagaaga aaaactgaca ggtgaccgat gtacaggact ttcctctaaa
     3421 atgcaagata caatggagga gaacagtgag agtgctctac ggaaacgtat tcgagaggac
     3481 agaaaggcca ccacagctca gaaggtgcag cagatgaaac agaggctaaa tgagaatgaa
     3541 cgaaaaagaa aaaggtggtt atattggcaa cctattctca ctaagatggg gtttatgtca
     3601 gtcattttgg ttggcgcttt tgttggctgg agactgtttt ttcagcaaaa tgccctataa
     3661 gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     3721 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3781 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3841 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3901 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3961 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4021 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4081 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     4141 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4201 atccagcgga ccttccttcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     4261 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     4321 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     4381 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     4441 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     4501 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     4561 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     4621 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     4681 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     4741 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     4801 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     4861 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     4921 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     4981 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     5041 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     5101 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     5161 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     5221 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     5281 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     5341 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     5401 agacgaagct tggtaccgag ctcggatcaa ttcgccacca tggtgagcaa gggcgaggag
     5461 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5521 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5581 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttgacctac
     5641 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5701 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5761 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5821 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     5881 agccacaagg tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag
     5941 acccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     6001 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     6061 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     6121 gccgggatca ctctcggcat ggacgagctg tacaagtaac tcgagggaat taattcgagc
     6181 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6241 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     6301 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6361 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6421 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6481 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     6541 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     6601 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     6661 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     6721 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     6781 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     6841 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     6901 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     6961 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     7021 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7081 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     7141 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     7201 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7261 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7321 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7381 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7441 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7501 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7561 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7621 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7681 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7741 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7801 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7861 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7921 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7981 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8041 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8101 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8161 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8221 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8281 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8581 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9121 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9181 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9241 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     9301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9361 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9421 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9481 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9541 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9601 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     9661 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     9721 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     9781 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     9841 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     9901 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     9961 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
    10021 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
    10081 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
    10141 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
    10201 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
    10261 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
    10321 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
    10381 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
    10441 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
    10501 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
    10561 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
    10621 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
    10681 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    10741 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
    10801 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
    10861 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
    10921 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
    10981 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
    11041 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
    11101 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
    11161 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
    11221 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
    11281 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
    11341 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
    11401 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11461 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    11521 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    11581 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    11641 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
    11701 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
    11761 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    11821 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    11881 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    11941 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    12001 ccggagtact tcaagaactg c
//
