LOCUS       sequence-398102-       11618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express Tyr to Phe mutant FAKSPARK2 in a lenti vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Li X, Combs JD 3rd, Salaita K, Shu X
  TITLE     Polarized focal adhesion kinase activity within a focal adhesion 
            during cell migration.
  JOURNAL   Nat Chem Biol. 2023 Jun 22. doi: 10.1038/s41589-023-01353-y.
  PUBMED    37349581
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Jun 22. doi: 10.1038/s41589-023-01353-y."
COMMENT     Sequence Label: lenti-fullef1a-mutFAKSPARK2
FEATURES             Location/Qualifiers
     source          1..11618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2763..3945
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2994..3936
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3893..3913
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3958..3967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4042..4044,4045..4047,4048..4758)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4087..4108)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4348..4367)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4695..4716
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4954..5007
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    6310..6898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6363..6383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6781..6792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6901..6917)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6902..6918)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7423..7603
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7629..7646)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7635..7859
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7905..8333
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7992..8011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8202..8223
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8342..8362)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8347..8676
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8527..8662
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8589..8608
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8724..8771
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8790..9164
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9294..9415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9331..9350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9385..9404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9464..9480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9464..9480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9477..9499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9488..9504
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9512..9518,9519..9536,9537..9542))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9557..9578
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9695..9712)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9866..10454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9946..9965)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10625..11416,11417..11485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11248..11267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11486..11590)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagccgat gagtaattca tacaaaagga ctcgcccctg
     2641 ccttggggaa tcccagggac cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct
     2701 gcgttcccgc cccctcaccc gcccgctctc gtcatcactg aggtggagaa gagcatgcgt
     2761 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2821 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2881 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2941 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     3001 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     3061 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     3121 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     3181 gcctggcttg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     3241 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     3301 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     3361 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     3421 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     3481 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3541 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3601 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3661 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3721 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3781 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3841 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3901 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgacccgg atctagcgcc
     3961 accatggaga cggatgattt cgcggagata atagatgagc tcgagtccgg actcagatct
     4021 ggcagcggtg gaggcgatat catggtgagc aagggcgagg agctgttcac cggggtggtg
     4081 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4141 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4201 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4261 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4321 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4381 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4441 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4501 atggccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     4561 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4621 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4681 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4741 atggacgagc tgtacaaggg atccggcagt gctggtggta gtgctggtgg tagtgctggt
     4801 ggtagtgctg gtggcagtgc tggtggtagt gctggtggta gtgctggtgg ctctagagga
     4861 gaaatcgcaa agagtctgaa agaaattgca aaatccttga aggaaatagc gtggtcactg
     4921 aaagaaattg ccaagtccct caaagggggt accgagggca gaggaagtct gctaacatgc
     4981 ggtgacgtcg aggagaatcc tggcccaaag cttgccacca tgtggtattt tggcaagatc
     5041 accagacggg agtcagagcg gttactgctc aatgcagaga acccgagagg gaccttcctc
     5101 gtgcgagaaa gtgagaccac gaaaggtgcc tactgcctct cagtgtctga cttcgacaac
     5161 gccaagggcc tcaacgtgaa gcactacaag atccgcaagc tggacagcgg cggcttctac
     5221 atcacctccc gcacccagtt caacagcctg cagcagctgg tggcctacta ctccaaacac
     5281 gccgatggcc tgtgccaccg cctcaccacc gtgtgcctcg agccgcggtc tggtctgcgt
     5341 tctggttctg gtggtggtat cgatatggtt tctaaaggtg aagaactgtt tactggtgtt
     5401 gtaccgatcc tggttgaact ggacggcgac gtaaatggtc ataaattctc cgtttctggt
     5461 gagggcgagg gtgatgcaac ttatggcaag ctgaccctga aattcatttg caccaccgga
     5521 aaactgccgg ttccgtggcc gaccctggtg accactctga cctatggcgt acagtgcttc
     5581 tctcgctacc cggatcacat gaaacagcac gactttttca aatctgcgat gccggaaggt
     5641 tacgtacaag agcgtaccat cttcttcaaa gatgacggta attacaaaac aagagcggaa
     5701 gttaaattcg agggtgacac cctggtgaac cgcatcgaac tgaaaggcat cgacttcaaa
     5761 gaggatggca acatcctggg ccacaaactg gaatacaact acaactccca caacgtgtac
     5821 atcatggctg ataagcagaa gaacggcatc aaagcgaact tcaaaattcg tcacaacatc
     5881 gaagacggct ctgttcaact ggcggatcac taccagcaga acacgccgat tggcgacggc
     5941 ccggttctgc tgccggataa ccactatctg tccacccagt ctgccctgtc taaagacccg
     6001 aacgagaaac gtgaccatat ggtactgctg gaatttgtta ccgccgctgg tatcaccctg
     6061 ggtatggacg aactgtacaa gaccggtggc tccgcaggtg gttccgctgg tggaagcgcc
     6121 ggtggttctg cgggtggatc tgctggaggt tctgcaggag gaagcgctgg aggtgggcgc
     6181 gccaccttga gagaaatcga ggaattgttg aggaaaatca ttgaagacag cgtgcgcagt
     6241 gtggccgagc tggaggacat tgagaagtgg ctgaagaaaa tctaagaatt cgatatcaag
     6301 cttatcggta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     6901 cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
     6961 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     7021 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7081 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
     7141 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
     7201 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     7261 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
     7321 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
     7381 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
     7441 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7501 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7561 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg
     7621 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7681 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7741 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7801 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7861 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     7921 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7981 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8041 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8101 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8161 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8221 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8281 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     8341 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8401 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8521 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8581 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     8641 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8701 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     8761 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     8821 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     8881 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     8941 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     9001 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     9061 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     9121 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     9181 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9241 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9421 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9541 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9601 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9661 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     9901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     9961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    10501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    10561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    10621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    10681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    10741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    10801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    10861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    10921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    10981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    11461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    11521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    11581 gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
