LOCUS       sequence-398106-       11937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human PTPN2 transcript variant 
            2 under the spleen focus-forming virus (SFFV) promoter and Emerald 
            under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11937)
  TITLE     PTPN2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLTR-SFFV-PTPN2 transcript variant
            2-WPRE-UbC-...
FEATURES             Location/Qualifiers
     source          1..11937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    305..430
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    927..1160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1345..1389
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1674..1791
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1944..2351
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(3642..3662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4060..4071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5110..5129
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5303..5312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5309..5311,5312..5314,5315..6028)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5354..5375)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5615..5634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5962..5983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6123..6356
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6381..6399)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6382..6399)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6689..6708)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6922..6941)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7041..7063
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7101..7119)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7187..7291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7292..7360,7361..8152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7510..7529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8323..8911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8812..8831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9065..9082
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9152..9172)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9157..9486
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9337..9472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10620..10685
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10815..10835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11260..11394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11297..11316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11351..11370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11562..11937,1..258)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
       61 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      241 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      301 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      361 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      421 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      481 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      541 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      601 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      661 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      721 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
      781 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
      841 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
      901 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
      961 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1021 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1081 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1141 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1201 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1261 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1321 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1381 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1441 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     1501 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     1561 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     1621 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
     1681 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1741 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     1801 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc
     1861 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     1921 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     1981 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     2041 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     2101 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     2161 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     2221 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     2281 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     2341 agtcgcccgg gggggatccg ccaccatgcc caccaccatc gagcgggagt tcgaagagtt
     2401 ggatactcag cgtcgctggc agccgctgta cttggaaatt cgaaatgagt cccatgacta
     2461 tcctcataga gtggccaagt ttccagaaaa cagaaatcga aacagataca gagatgtaag
     2521 cccatatgat cacagtcgtg ttaaactgca aaatgctgag aatgattata ttaatgccag
     2581 tttagttgac atagaagagg cacaaaggag ttacatctta acacagggtc cacttcctaa
     2641 cacatgctgc catttctggc ttatggtttg gcagcagaag accaaagcag ttgtcatgct
     2701 gaaccgcatt gtggagaaag aatcggttaa atgtgcacag tactggccaa cagatgacca
     2761 agagatgctg tttaaagaaa caggattcag tgtgaagctc ttgtcagaag atgtgaagtc
     2821 gtattataca gtacatctac tacaattaga aaatatcaat agtggtgaaa ccagaacaat
     2881 atctcacttt cattatacta cctggccaga ttttggagtc cctgaatcac cagcttcatt
     2941 tctcaatttc ttgtttaaag tgagagaatc tggctccttg aaccctgacc atgggcctgc
     3001 ggtgatccac tgtagtgcag gcattgggcg ctctggcacc ttctctctgg tagacacttg
     3061 tcttgttttg atggaaaaag gagatgatat taacataaaa caagtgttac tgaacatgag
     3121 aaaataccga atgggtctta ttcagacccc agatcaactg agattctcat acatggctat
     3181 aatagaagga gcaaaatgta taaagggaga ttctagtata cagaaacgat ggaaagaact
     3241 ttctaaggaa gacttatctc ctgcctttga tcattcacca aacaaaataa tgactgaaaa
     3301 atacaatggg aacagaatag gtctagaaga agaaaaactg acaggtgacc gatgtacagg
     3361 actttcctct aaaatgcaag atacaatgga ggagaacagt gagagtgctc tacggaaacg
     3421 tattcgagag gacagaaagg ccaccacagc tcagaaggtg cagcagatga aacagaggct
     3481 aaatgagaat gaacgaaaaa gaaaaaggcc aagattgaca gacacctaag cggccgcgac
     3541 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
     3601 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3661 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3721 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3781 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3841 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3901 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3961 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4021 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4081 cttccttccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     4141 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     4201 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     4261 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     4321 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     4381 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     4441 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     4501 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     4561 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     4621 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     4681 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     4741 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     4801 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     4861 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     4921 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     4981 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     5041 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     5101 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     5161 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     5221 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     5281 ggtaccgagc tcggatcaat tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     5341 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5401 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5461 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg gcgtgcagtg
     5521 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5581 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5641 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5701 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaaggt
     5761 ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga cccgccacaa
     5821 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5881 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5941 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6001 tctcggcatg gacgagctgt acaagtaact cgagggaatt aattcgagct cggtaccttt
     6061 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6121 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6181 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6241 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6301 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     6361 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     6421 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     6481 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     6541 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     6601 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6661 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6721 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6781 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6841 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6901 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     6961 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7021 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7081 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7141 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7201 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7261 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7321 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7381 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7441 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7501 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7561 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7621 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7681 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7741 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7801 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7861 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7921 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7981 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8041 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8101 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8161 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8221 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8281 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8341 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8401 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8461 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8521 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8581 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8641 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8701 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8761 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8821 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8881 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8941 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9001 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9061 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9121 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     9181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9241 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9301 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9481 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     9541 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     9601 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     9661 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     9721 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     9781 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     9841 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     9901 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     9961 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    10021 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    10081 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    10141 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    10201 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    10261 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    10321 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    10381 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
    10441 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    10501 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
    10561 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    10621 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
    10681 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
    10741 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
    10801 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
    10861 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
    10921 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
    10981 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
    11041 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
    11101 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
    11161 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
    11221 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
    11281 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11341 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    11401 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    11461 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    11521 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
    11581 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    11641 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
    11701 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    11761 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    11821 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    11881 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagg
//
