LOCUS       sequence-398121-       11476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express mCherry-BRCA1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Li X, Chung CI, Yang J, Chaudhuri S, Munster PN, Shu X
  TITLE     ATM-SPARK: A GFP phase separation-based activity reporter of ATM.
  JOURNAL   Sci Adv. 2023 Mar;9(9):eade3760. doi: 10.1126/sciadv.ade3760. Epub 
            2023 Mar 1.
  PUBMED    36857446
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03"; volume: "9(9):eade3760. doi"; pages: " 
            10.1126/sciadv.ade3760. Epub 2023 Mar 1"
COMMENT     Sequence Label: pcDNA-BRCA1-mCherry
FEATURES             Location/Qualifiers
     source          1..11476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1697
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1135..1153)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1403..1422
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1707..1731
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7328..7352)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7352..7376)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7428..7445)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7434..7658
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7791..7810)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7942..7989
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7997..9019
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9149..9270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9186..9205)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9240..9259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9319..9335)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9319..9335)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9332..9354)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9343..9359
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9367..9373,9374..9391,9392..9397))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9412..9433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9550..9567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9721..10309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9801..9820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10480..11271,11272..11340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11103..11122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11341..11445)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag gataacatgg
     1021 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1141 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1201 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1321 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1381 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1441 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1501 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1561 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1621 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1681 tggacgagct gtacaagggc gcgcccacaa gtttgtacaa aaaagcaggc tccaccatgg
     1741 atttatctgc tcttcgcgtt gaagaagtac aaaatgtcat taatgctatg cagaaaatct
     1801 tagagtgtcc catctgtctg gagttgatca aggaacctgt ctccacaaag tgtgaccaca
     1861 tattttgcaa attttgcatg ctgaaacttc tcaaccagaa gaaagggcct tcacagtgtc
     1921 ctttatgtaa gaatgatata accaaaagga gcctacaaga aagtacgaga tttagtcaac
     1981 ttgttgaaga gctattgaaa atcatttgtg cttttcagct tgacacaggt ttggagtatg
     2041 caaacagcta taattttgca aaaaaggaaa ataactctcc tgaacatcta aaagatgaag
     2101 tttctatcat ccaaagtatg ggctacagaa accgtgccaa aagacttcta cagagtgaac
     2161 ccgaaaatcc ttccttgcag gaaaccagtc tcagtgtcca actctctaac cttggaactg
     2221 tgagaactct gaggacaaag cagcggatac aacctcaaaa gacgtctgtc tacattgaat
     2281 tgggatctga ttcttctgaa gataccgtta ataaggcaac ttattgcagt gtgggagatc
     2341 aagaattgtt acaaatcacc cctcaaggaa ccagggatga aatcagtttg gattctgcaa
     2401 aaaaggctgc ttgtgaattt tctgagacgg atgtaacaaa tactgaacat catcaaccca
     2461 gtaataatga tttgaacacc actgagaagc gtgcagctga gaggcatcca gaaaagtatc
     2521 agggtagttc tgtttcaaac ttgcatgtgg agccatgtgg cacaaatact catgccagct
     2581 cattacagca tgagaacagc agtttattac tcactaaaga cagaatgaat gtagaaaagg
     2641 ctgaattctg taataaaagc aaacagcctg gcttagcaag gagccaacat aacagatggg
     2701 ctggaagtaa ggaaacatgt aatgataggc ggactcccag cacagaaaaa aaggtagatc
     2761 tgaatgctga tcccctgtgt gagagaaaag aatggaataa gcagaaactg ccatgctcag
     2821 agaatcctag agatactgaa gatgttcctt ggataacact aaatagcagc attcagaaag
     2881 ttaatgagtg gttttccaga agtgatgaac tgttaggttc tgatgactca catgatgggg
     2941 agtctgaatc aaatgccaaa gtagctgatg tattggacgt tctaaatgag gtagatgaat
     3001 attctggttc ttcagagaaa atagacttac tggccagtga tcctcatgag gctttaatat
     3061 gtaaaagtga aagagttcac tccaaatcag tagagagtaa tattgaagac aaaatatttg
     3121 ggaaaaccta tcggaagaag gcaagcctcc ccaacttaag ccatgtaact gaaaatctaa
     3181 ttataggagc atttgttact gagccacaga taatacaaga gcgtcccctc acaaataaat
     3241 taaagcgtaa aaggagacct acatcaggcc ttcatcctga ggattttatc aagaaagcag
     3301 atttggcagt tcaaaagact cctgaaatga taaatcaggg aactaaccaa acggagcaga
     3361 atggtcaagt gatgaatatt actaatagtg gtcatgagaa taaaacaaaa ggtgattcta
     3421 ttcagaatga gaaaaatcct aacccaatag aatcactcga aaaagaatct gctttcaaaa
     3481 cgaaagctga acctataagc agcagtataa gcaatatgga actcgaatta aatatccaca
     3541 attcaaaagc acctaaaaag aataggctga ggaggaagtc ttctaccagg catattcatg
     3601 cgcttgaact agtagtcagt agaaatctaa gcccacctaa ttgtactgaa ttgcaaattg
     3661 atagttgttc tagcagtgaa gagataaaga aaaaaaagta caaccaaatg ccagtcaggc
     3721 acagcagaaa cctacaactc atggaaggta aagaacctgc aactggagcc aagaagagta
     3781 acaagccaaa tgaacagaca agtaaaagac atgacagtga tactttccca gagctgaagt
     3841 taacaaatgc acctggttct tttactaagt gttcaaatac cagtgaactt aaagaatttg
     3901 tcaatcctag ccttccaaga gaagaaaaag aagagaaact agaaacagtt aaagtgtcta
     3961 ataatgctga agaccccaaa gatctcatgt taagtggaga aagggttttg caaactgaaa
     4021 gatctgtaga gagtagcagt atttcactgg tacctggtac tgattatggc actcaggaaa
     4081 gtatctcgtt actggaagtt agcactctag ggaaggcaaa aacagaacca aataaatgtg
     4141 tgagtcagtg tgcagcattt gaaaacccca agggactaat tcatggttgt tccaaagata
     4201 atagaaatga cacagaaggc tttaagtatc cattgggaca tgaagttaac cacagtcggg
     4261 aaacaagcat agaaatggaa gaaagtgaac ttgatgctca gtatttgcag aatacattca
     4321 aggtttcaaa gcgccagtca tttgctctgt tttcaaatcc aggaaatgca gaagaggaat
     4381 gtgcaacatt ctctgcccac tctgggtcct taaagaaaca aagtccaaaa gtcacttttg
     4441 aatgtgaaca aaaggaagaa aatcaaggaa agaatgagtc taatatcaag cctgtacaga
     4501 cagttaatat cactgcaggc tttcctgtgg ttggtcagaa agataagcca gttgataatg
     4561 ccaaatgtag tatcaaagga ggctctaggt tttgtctatc atctcagttc agaggcaacg
     4621 aaactggact cattactcca aataaacatg gacttttaca aaacccatat cgtataccac
     4681 cactttttcc catcaagtca tttgttaaaa ctaaatgtaa gaaaaatctg ctagaggaaa
     4741 actttgagga acattcaatg tcacctgaaa gagaaatggg aaatgagaac attccaagta
     4801 cagtgagcac aattagccgt aataacatta gagaaaatgt ttttaaagga gccagctcaa
     4861 gcaatattaa tgaagtaggt tccagtacta atgaagtggg ctccagtatt aatgaaatag
     4921 gttccagtga tgaaaacatt caagcagaac taggtagaaa cagagggcca aaattgaatg
     4981 ctatgcttag attaggggtt ttgcaacctg aggtctataa acaaagtctt cctggaagta
     5041 attgtaagca tcctgaaata aaaaagcaag aatatgaaga agtagttcag actgttaata
     5101 cagatttctc tccatatctg atttcagata acttagaaca gcctatggga agtagtcata
     5161 catctcaggt ttgttctgag acacctgatg acctgttaga tgatggtgaa ataaaggaag
     5221 atactagttt tgctgaaaat gacattaagg aaagttctgc tgtttttagc aaaagcgtcc
     5281 agagaggaga gcttagcagg agtcctagcc ctttcaccca tacacatttg gctcagggtt
     5341 accgaagagg ggccaagaaa ttagagtcct cagaagagaa cttatctagt gaggatgaag
     5401 agcttccctg cttccaacac ttgttatttg gtaaagtaaa caatatacct tctcagtcta
     5461 ctaggcatag caccgttgct accgagtgtc tgtctaagaa cacagaggag aatttattat
     5521 cattgaagaa tagcttaaat gactgcagta accaggtaat attggcaaag gcatctcagg
     5581 aacatcacct tagtgaggaa acaaaatgtt ctgctagctt gttttcttca cagtgcagtg
     5641 aattggaaga cttgactgca aatacaaaca cccaggatcc tttcttgatt ggttcttcca
     5701 aacaaatgag gcatcagtct gaaagccagg gagttggtct gagtgacaag gaattggttt
     5761 cagatgatga agaaagagga acgggcttgg aagaaaataa tcaagaagag caaagcatgg
     5821 attcaaactt aggtgaagca gcatctgggt gtgagagtga aacaagcgtc tctgaagact
     5881 gctcagggct atcctctcag agtgacattt taaccactca gcagagggat accatgcaac
     5941 ataacctgat aaagctccag caggaaatgg ctgaactaga agctgtgtta gaacagcatg
     6001 ggagccagcc ttctaacagc taccctagta taatcagtga ctcttctgcc cttgaggacc
     6061 tgcgaaatcc agaacaaagc acatcagaaa aagcagtatt aacttcacag aaaagtagtg
     6121 aataccctat aagccagaat ccagaaggcc tttctgctga caagtttgag gtgtctgcag
     6181 atagttctac cagtaaaaat aaagaaccag gagtggaaag gtcatcccct tctaaatgcc
     6241 catcattaga tgataggtgg tacatgcaca gttgctctgg gagtcttcag aatagaaact
     6301 acccatctca agaggagctc attaaggttg ttgatgtgga ggagcaacag ctggaagagt
     6361 ctgggccaca cgatttgacg gaaacatctt acttgccaag gcaagatcta gagggaaccc
     6421 cttacctgga atctggaatc agcctcttct ctgatgaccc tgaatctgat ccttctgaag
     6481 acagagcccc agagtcagct cgtgttggca acataccatc ttcaacctct gcattgaaag
     6541 ttccccaatt gaaagttgca gaatctgccc agggtccagc tgctgctcat actactgata
     6601 ctgctgggta taatgcaatg gaagaaagtg tgagcaggga gaagccagaa ttgacagctt
     6661 caacagaaag ggtcaacaaa agaatgtcca tggtggtgtc tggcctgacc ccagaagaat
     6721 ttatgctcgt gtacaagttt gccagaaaac accacatcac tttaactaat ctaattactg
     6781 aagagactac tcatgttgtt atgaaaacag atgctgagtt tgtgtgtgaa cggacactga
     6841 aatattttct aggaattgcg ggaggaaaat gggtagttag ctatttctgg gtgacccagt
     6901 ctattaaaga aagaaaaatg ctgaatgagc atgattttga agtcagagga gatgtggtca
     6961 atggaagaaa ccaccaaggt ccaaagcgag caagagaatc ccaggacaga aagatcttca
     7021 gggggctaga aatctgttgc tatgggccct tcaccaacat gcccacagat caactggaat
     7081 ggatggtaca gctgtgtggt gcttctgtgg tgaaggagct ttcatcattc acccttggca
     7141 caggtgtcca cccaattgtg gttgtgcagc cagatgcctg gacagaggac aatggcttcc
     7201 atgcaattgg gcagatgtgt gaggcacctg tggtgacccg agagtgggtg ttggacagtg
     7261 tagcactcta ccagtgccag gagctggaca cctacctgat accccagatc ccccacagcc
     7321 actactgaac ccgctttctt gtacaaagtg gtttcagctt tcttgtacaa agtggtgtga
     7381 ctcgagctga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     7441 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7501 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7561 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     7621 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     7681 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     7741 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7801 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7861 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7921 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     7981 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     8041 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     8101 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     8161 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     8221 agtgcttgac attggggatt tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     8281 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     8341 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     8401 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     8461 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     8521 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     8581 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     8641 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     8701 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     8761 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     8821 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     8881 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     8941 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     9001 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     9061 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9121 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9181 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9241 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9301 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9361 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9421 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9481 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10141 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11461 agtgccacct gacgtc
//
