LOCUS       sequence-398129-       10667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains the entire replication competent Sindbis genome 
            with the structural genome components replaced by EGFP in an MCS 
            locus. See Resource Information section for annotated plasmid 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh
            D, Strachan RT, Wacker D, Roth BL
  TITLE     VEGAS as a Platform for Facile Directed Evolution in Mammalian 
            Cells.
  JOURNAL   Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 
            10.1016/j.cell.2019.05.051.
  PUBMED    31280962
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051."
COMMENT     Sequence Label: pTSin EGFP
FEATURES             Location/Qualifiers
     source          1..10667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     185..204
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     444..465
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      507..516
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8197..8215)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8198..8215)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     rep_origin      complement(8303..8891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8383..8402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9062..9853,9854..9922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9685..9704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9923..10027)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(join(10458..10667,1..504,505..507,508..510))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(10461..10667,1..504,505..507,508..510))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10503..10524)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
       61 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      121 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      181 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      241 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
      301 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      361 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      421 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      481 ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtgatcgat gcggccgctg
      541 gtggtgttgt ggtattagtc agatgaaatg tactatgctg actatttagg accaccgtag
      601 agatgcttta tttcccctct gatggcttga aatgctcttt tgctctgggc aaaagttctc
      661 aatgccagca ggacaggtgt gatgttgtct acctcatacc gagttgccac ggccactgct
      721 aaggtgtctg ttatacctac tctaaaccac gcctttgttt catctagcag agcgcgtctt
      781 ctgtcttcgt cttgctcatc gtcggctggg agcggtttac ccaacttaaa cagccttttc
      841 aaggggtccg ccacgcgaca cgctgtggag gtaaccgaat cttgcaagat gaatccaccg
      901 cagaagtaag gcggtctctc gccgatgact gcgtcaatga tcttaacctc catgttgagc
      961 caggtggcac acctctcagc catttctttg tcagatacta ctccgtgtat gatgttgtcg
     1021 tcgccgataa atgctgcaca tttggacgtt ttaagccgct cctccaatac tctgctggcg
     1081 ataacgacat tcagaactgt gttgacaaag agcgtgagga acattccgga tttcatcatc
     1141 gccccgaatt tgaaacgggt acccgtgggc agatgggtgg atgatatttc tccaaaggcg
     1201 cactcgatca agtcgagtag tggttggtcc acacccaggt cttccaagat catcaggcca
     1261 gttaacgcca tagcgtcgtc ttggcttttg tcgaacgagg cgatatccgt ctccagtacc
     1321 gggtcacctt gcttgaagtg ttctgctatg attgcatcaa agtcctccgc cgacatgtca
     1381 aaaagcgtgt gaatgttggg tagcaagacg gctgtaagcc tgcgcactaa ctcccggtgg
     1441 atcccgcaca ggtaagcggt cgccaggggt tctgcggctt gtatcacttg tactttcggt
     1501 ctttcttctg tgtgtttcgt gccaggtgta actttcacgt ctcttttcat gtccatgacg
     1561 aacctatcca taggcacttc ttgcaatggg accaaattat gcgtctttgc gaacagtgcg
     1621 gcggccttag ggcctttcag tctggccacg tatgcggtaa cgaactcagt agtgatccta
     1681 attggctttc gggcaaactc ctcccaatac tcgtcattac atgcatattt tcgaaagcat
     1741 tcaacgttga atgtcgctga gtccagtgtt ggcaattcac gcatttgtgt gacgttgcag
     1801 tttcttttag tcgcggcaat gagcacgttt tgcaacgtgt tctgcatcgc tgatggaacc
     1861 gcactgcgga tgtttggggc tctatactcg tgtcttttcg ggtaacttct aagcttggcg
     1921 gggcaaaaag ttgcagtatc taggcaagcg actgtcccgt ctaccatatc caagtaagca
     1981 tcgtactcgt cggtgatctg ataagatgct accgtcgggt aattctcatg cagatagttg
     2041 ttgcaaacag ctacagcaaa ctttgggtca gagtagttcg ccggtacact gctggaatac
     2101 gatggtttcg ggtaggtgat cttatagcat tctggctgat ctgtggcaga gttatacagt
     2161 cgtagccctg aaagcagtcg ctcagtggtt atggctttct gattttctac ttttctggac
     2221 tggtacctgc ttttgttggc ttcggtgggc atcatctggt acctgagttt gagctgttcc
     2281 tctttcgacg tgtcgagcac cggggcgtag attctttcca gaacattgcg ctccaaggtc
     2341 ggttctgtaa gctggttctg cagaacggac ttcttttgca agtgcccagg gcctgtgtcc
     2401 gtcgaaaata tgtacccacc taccccggtt agtcagtatt cggtcctcct gctcctgcgt
     2461 ctacgtctct gcttgcgtgg tggaaaagat acggctgatc gggacgatat aattgagttc
     2521 acttcgcccg gttcaaatga cccaaacagg acgggctcag actcggttac tctgcggctc
     2581 agctcctcaa tctctccgtc ggaaaacgat ccgaaagaca taggcacatc cgtagggcat
     2641 gtacttgccg ggggttgtgc cgctgccaag cgggccatct ctccgtcgaa aagggatccg
     2701 aaggacatgg ataccccacc aaaagaaagg tgaagggact cgtccgcaga gctggtgctt
     2761 gccggtggag ttggctcttc ctgcattctt gccgctgcca ggcgggccat cttctttagc
     2821 cttggcggtg gaacaggggc aggctcttgg acggcatgga cgtcagccac caccacctgc
     2881 cttcggtcta ctatctctag tgaactaggt cctgacgacc aactgtccat actagtaata
     2941 gagttgtccg atccgctaaa gctcgaaaag agtgagcctt cgctactgtc ttccatgtcc
     3001 agtgagatgt ccgtgacatc aagcgaggtg ttatcagctg caggtggtgt tggtgttgct
     3061 gcaacttcgg gggcctcctc ggcctgtgca ggcggagctg caggctgttc tggcgcttct
     3121 atgtacttac gggcgggaac gaatgcaggg gtatgcgggt taaacaggac tacttttgtg
     3181 cactgaacct tctgaacgtt cttgattttg tactttggaa ggggggtgga ggagcatact
     3241 gtaacttctt tgacgttgtt gcttctgagt ctgtggaccc tttctggcgt catggcatac
     3301 atgcagaggc acggcagcgt ttttggcggg ctagacgacg ggttgtggtc gaccgggcat
     3361 ttttcgcgga ttgcttccat ggtctccccc aatatgtagg cacacagttg ctcgttgctt
     3421 tcctggtcat ttgggaacag gacctttatc tccgccatat cttttgctgc ttgatggaat
     3481 ttggtgcctt caaagtacga atacaacttt ccttttgtag tactgaatcc ctttcttccc
     3541 ttcaggcaac tgtccggatg gatccatact aactcgtcgt cgatctccat atcctcatcc
     3601 ttcagctctg ttacagactc cttaagttgg agcaccgcgt cgattctttc cttccacttc
     3661 ttatccaggc agtagatggt tacgtccgca tcagttctat ctagcgcggt tgtcaagcag
     3721 ttaagtgata cttcaaggcg gtcttttccg gctgcgtaaa tgcctgtaga tagcagtggg
     3781 atggcgacag acttgatatt atgttcattt actaagtctg ccactgcatg gtaggcgttt
     3841 tgcagcaatt tcagggcttc tgcctctggg tgtttccgga aatcagggcc aaccgcgtgg
     3901 atcactttct ttccttggca cacagtcagt tttgcggtgc cggtctctgt ggctgaatcg
     3961 gtgaaactgt tcggccaacg tttatagatg gcacggcaga ctccttcgcc tggtctgccc
     4021 agcggattgg ctgcattgac aactgcttcc tcttgacaat cagcaatgtt ctccctttta
     4081 gtgcggtatg acggtgcggc tccaactccg tctcttgtac cctcgtacac ggacgaaatc
     4141 acacaattca gatgatgcgg ggtgaattgt cgtgtgcggc tgttgtctag ttgtcggaag
     4201 atcaggtaca tttctgtatt gcttgagacg cactctggcc tcgctgcaga cactctgaca
     4261 aattttctgg caagagcggt gactacgtcc tcactattgc ggtcggcgta accgtaggac
     4321 ttcaccacga gggtgcctcc ggggttaagg cagttcaggg ccgaacgcga gagggttttc
     4381 aaggtcgccg catggtcttc gcactgctga aagtgatggt ttctgtattt agttccaata
     4441 ttgataaaca ccaggtcgta ccgtgcctgc ggcggaaacc cgaaagccag gttgtagttc
     4501 ttatcagcgc cggctatgcc aatcggggcg atccattcga ttctcttgtg gggagcttca
     4561 attttttcct ctgagaccac aagtacggag tggtgtttga actggctcaa gaattttttg
     4621 accgggccgg gttgtttctc cttgtgctcg gggactaagg cgtgcgggag attgcggttc
     4681 actgggacca agttatgctg tgcggagata actctagttc tgcccgtctg caaatcaagc
     4741 tgtgtgcctt tcccagctag ctggaacacc ggaaatctac gggagagttc ggcggcaacg
     4801 gcgtgatcgt acccatactt gcgggttcct gggctgttgt cccaatgagc tactggcctc
     4861 gctgaatcgg caggatggta cgttaacggg atgctctgtt tggaaaacag tccgcttgtc
     4921 aagtccatgc cgaaaaactt aatgcagatt acgtccaggg cgtagatggc cgagtgtggt
     4981 ttgtcatctg caaactgtgg gaacagctcg ctccactggc aaccggtaag tacgataccg
     5041 gccgtggcca gtatcggttc cagtgctttc gcccagcaaa cgttagtctt gcagctgaac
     5101 ggattggtac ggggagcggg actgtttatc gcagcaatta ttcccttgtg ttcagcttcc
     5161 cagtcctcga tggtggcttg aaaatttcct tttggtacgt tagtgagctg cttaatccat
     5221 gggtcgccct gtaaagtttt ccatactagc ctgtcctcag tgcgggtgag cagcacgttc
     5281 acatgctctg atgtgatcgc gtacagcggg ttttcattga ctttttgccg gacggcatat
     5341 actccttttc tggttagccc ttgtgaggcc gcggctgtca ttacctcatg tccgggatag
     5401 tcgatttgca gttgcttaac ccacccgcgg aagcatgtca ggatgatgtc ccctggcttc
     5461 ggcttcgtgg cccctgtaat gtcgatttcg atgttcttct tgcacgggtt tgtggttttc
     5521 atttttccat cgtaatgcag tgtcgataca atagccgtga ctggctgtgt gcaacgtcgg
     5581 gagataaact tgtagaatgt cttggtacat atgtcttttt ccgggtggtt gaaatatacc
     5641 tttagttgca tcatgttgaa gaatccgcat tgcttagggt ctccgcatag cactaccttc
     5701 ttacggggtc tgacgattgc aatcaaggca agtagtgctc ctgcgtggca cgcgaacgct
     5761 tcgtcaacat acagcacttc tacggctttg tggcatccgt tgagcataac cgaatccact
     5821 gtcttcgacg tgatctgcat gcccctcagc cgtagcacat cggcctcaat ttcgcggcag
     5881 ttttctttct ttccgctggt aacaagatca cgtgccgtga cagttgactt gatgatagcc
     5941 gacttgcccg atcctggtgt gcctatcact cctattgttt caaccttgta cgggaccgcg
     6001 ggtcgagtct tcagtccctc aagagctagt tcgtgatagg gcgggttggt cagttctccc
     6061 gagaggacaa gtcctgaggc ttcttccttc ttgacgcatc gcttcttgtc cacgtcaaac
     6121 acgtactctg tttctgcgag ctctgccttt gtaaccttgt actgctcctc ttctgtattc
     6181 ttagcgggac cgtgcatggc aatatggtac agcttgcggt tcacaaactc tctttcgttg
     6241 tacactagcg tggcgctctc actcagtgct aagaattctg gccatggtac ggcacttcct
     6301 gctggcatca gtactttagc gtcgtatggt tcgactgcat accttcctga tcttccggag
     6361 tgcgttatga tcttaacctg gtctgctagc gggtgtgctg gtgcgagttt agcgttcttc
     6421 agcacagagg ttggcgagac aacgatgtac tgtccgatca tacggtcatt tgcttgaggt
     6481 attatcctta catgaccgcg cggggtttcg acgagtgctg ctccgatgtc cgcctggagc
     6541 ccctccactt cgcagacaac ttccgcggct gcctcgatac ctttgtctgc cactaatggt
     6601 gggagtgctt ctcggagctt ctccgctctg gattcctcct gagcatcctc gaaagcagcc
     6661 ttggcctcca tgactaattc ctccgggact tgcagcagtt tttcctcctt ctttggttgt
     6721 aatgccaatt ttatcttctg cctcagcgac atgggcaaag aggtagtcca tacggatgac
     6781 atggggaaag cgctaaaaga ggctgggact tttacgatgg tctgcgttcc aggtgggcga
     6841 tagaacgagt gcactttctt agtgcgaaac gcccacaagc agccatatgt aagcttgcgc
     6901 tctctggtac ccagcatttt ttcattgtca aggtcttctt tgcgctcctt ggcccatttg
     6961 ctgaaccctt gtgcaatgat tggcagaagg taattttgca tggtattggt gttcctgtta
     7021 gtcttaccgt taatgacgat tcgctggttg agcccaacca gaagtttttg tgcatcgtca
     7081 ggtgagatat ccgtggccat tatgccggtc atctgatcgc atatggtggc cgggatatac
     7141 gtgcacacgg ggaacgatac ccgttctcct tttactgtat cggtaacttt gcatagcaag
     7201 aagccctcgc tattgtttgt aaccgcgtat cccacggttt ctcccgtgat cccgggactg
     7261 atggtgattt tcttcactac gtagccttcg cagctcacca ctgtatcaca gcggcaagtg
     7321 tacgactgct ttcctttcag gtggaacacc gatggaagat gccagctctg caagctggct
     7381 ctgtgttctg ggtaaagtgt cgatccaacg gagaaataaa cccgtgaccc gggcttcaac
     7441 tccttcttcc tcattatcga caactttcct gtcctgcctt cactcagctt tgtgctgcag
     7501 agtccgatgt tacgcgcttc gaggactttt tcgtcggccc agttggtgtt gtacgcaggg
     7561 tacgaacctg ccatagccga gaacatgaac tgggtggtat cgaagccaat ccagtacagg
     7621 gtccgcacgc ctttcatagc ctgatggtaa atagttccgg gtgcgttgat gtacacgtcc
     7681 tgcatgacgg agtactcggc acgcgtgttg caggtaacat cgttgtggaa gcagagtgat
     7741 ggcgtttcag catccggtgt atcaagtacg gtccggaggt ccttgatctt ctcatgcaag
     7801 ttcttattcg taatcttgca tgctttttcc gccagtttgc tggcatattt catcatgcgg
     7861 tccgggtctt ctggactacg catggggcaa acgcaatggt actggtgctc ggaaaacatt
     7921 ctacgagccg gtgcgctgcc tatgtccaaa atcgtcgctg tggtaggaac ctccagctcg
     7981 attagtttac tggccagatg cgaaaatgct ctggcattag catggtcatt tggagtgacc
     8041 tgctgtgcta ctacctcaaa ttgcgggaag ctcttttgca gttgcacgac aaacggactc
     8101 tgcgggtcta cgtctacgtt aactactggc ttctccattg tgatggtagt gcaattggtc
     8161 ggctgtttga ttcaatagtg tgtactacgc cgccaatcta tagtgtcacc taaatcgtat
     8221 gtgtatgata cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtccat
     8281 acgagctcgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9661 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10021 ttccgcgcac atttccccga aaagtgccac ctggcccttt cgtcttcaag aattaattcc
    10081 cctcgagctc tagagctcga gatccccttt tttttttttt tttttngaaa tgttaaaaac
    10141 aaaattttgt tgattaataa aagaaataat aaaaagttat gcagacgctg catggcatta
    10201 tgcaccatgc ttcctcagaa atacattgag ttttggcgtc cgctgaataa atggttaata
    10261 tagtgattat ttggcaacac tgcgcagcat tatgcactgc gcctcctcgg aaatacgtcg
    10321 agttttggtg ttgctatatt gcccgcggta agcggggatc taatatacca gcctgatgca
    10381 ttatgcacat cagttcctcg gaagtacatc gagttttgct ggtcgggtca ttggggcgta
    10441 ctagtatcga tgccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
    10501 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
    10561 gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
    10621 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgtt
//
