LOCUS       sequence-398130-        4344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-UNC93B1_STOP
FEATURES             Location/Qualifiers
     source          1..4344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1901..2000)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2017..2036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2018..2036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2041..2057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2041..2057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2159..2178)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2170..2979
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2237..2256)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3126..3714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3615..3634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3868..3885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4044..4071)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4163..4249)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4298..4320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4312..4329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4313..4329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga ggccgagccc
      121 cctctgtacc caatggcagg agcagcagga cctcagggcg acgaggatct gctgggagtg
      181 cctgatggac cagaggcacc actggacgag ctggtgggag cataccccaa ctataatgag
      241 gaggaggagg agcggagata ctataggcgc aagagactgg gcgtgctgaa gaacgtgctg
      301 gcagcatctg ccggaggaat gctgacctac ggcgtgtatc tgggcctgct gcagatgcag
      361 ctgatcctgc actatgatga gacatacagg gaggtgaagt atggcaacat gggcctgcct
      421 gacatcgata gcaagatgct gatgggcatc aatgtgaccc caatcgccgc cctgctgtac
      481 acacccgtgc tgatcagatt ctttggcacc aagtggatga tgttcctggc cgtgggcatc
      541 tatgccctgt ttgtgagcac caactactgg gagaggtact atacactggt gccttccgcc
      601 gtggccctgg gaatggcaat cgtgccactg tgggcctcca tgggcaatta catcacacgc
      661 atggcccaga agtaccacga gtattctcac tacaaggagc aggatggaca gggaatgaag
      721 cagaggccac caaggggatc ccacgcacca tatctgctgg tgttccaggc catcttttac
      781 agcttctttc acctgtcctt cgcctgcgca cagctgccaa tgatctattt tctgaaccac
      841 tacctgtatg acctgaacca caccctgtac aatgtgcagt cttgtggcac aaacagccac
      901 ggcatcctgt ccggcttcaa taagaccgtg ctgagaacac tgcctaggtc tggcaatctg
      961 atcgtggtgg agagcgtgct gatggccgtg gcctttctgg ccatgctgct ggtgctggga
     1021 ctgtgcggag cagcctatcg cccaaccgag gagatcgatc tgcggagcgt gggctggggc
     1081 aatatcttcc agctgccctt taagcacgtg cgcgactatc ggctgagaca cctggtgcct
     1141 ttctttatct actccggctt cgaggtgctg tttgcatgca caggaatcgc cctgggatac
     1201 ggcgtgtgct ctgtgggact ggagcggctg gcctatctgc tggtggccta cagcctgggc
     1261 gcctctgccg ccagcctgct gggactgctg ggactgtggc tgccaaggcc tgtgccactg
     1321 gtggccggcg ccggcgtgca cctgctgctg accttcatcc tgttcttttg ggcaccagtg
     1381 cctcgggtgc tgcagcactc ctggatcctg tatgtggcag ccgccctgtg gggagtggga
     1441 tccgccctga acaagaccgg cctgtctaca ctgctgggca tcctgtacga ggataaggag
     1501 agacaggact tcatctttac catctatcac tggtggcagg ccgtggccat cttcacagtg
     1561 tacctgggca gctccctgca catgaaggcc aagctggccg tgctgctggt gaccctggtg
     1621 gcagccgccg tgagctacct gaggatggag cagaagctga ggagaggagt ggcacctagg
     1681 cagccaagaa tcccaaggcc acagcacaag gtgcgcggat acaggtatct ggaggaggac
     1741 aattccgatg agtctgacgc cgagggcgag cacggcgatg gcgccgagga ggaggcccct
     1801 ccagccggcc ctaggccagg accagagcct gcaggactgg gcaggcgccc atgtccatac
     1861 gagcaggcac agggcggcga cggccccgag gagcagtaag acccagcttt cttgtacaaa
     1921 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1981 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2041 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2101 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     2161 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
     2221 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     2281 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     2341 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     2401 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     2461 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     2521 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     2581 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     2641 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     2701 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     2761 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2821 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2881 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2941 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
     3001 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
     3061 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3901 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3961 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4021 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4081 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4141 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4201 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4261 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4321 acggccagtc ttaagctcgg gccc
//
