LOCUS       sequence-398138-        2483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_297-370.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_297-370
FEATURES             Location/Qualifiers
     source          1..2483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(129..228)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      281..367
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      459..486
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(621..1209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(701..720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1352..2161)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2075..2094
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2153..2172
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2284..2383
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttctggatg tgcttctgca gctttgccag caagttaatc gccagcttga ttttctggaa
       61 gcagtcgatg aactcgccct cagagggggg ccgtgcccgc agtgtgagga cgccctctgc
      121 tggcatggag cctgcttttt tgtacaaagt tggcattata aaaaagcatt gctcatcaat
      181 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttggg gcccgagatc
      241 catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
      301 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      361 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc
      421 aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc tgacggatgg
      481 cctttttgcg tttctacaaa ctcttcctgg ctagcggtaa tacggttatc cacagaatca
      541 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
      841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
      961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1261 tcacgttaag ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct
     1321 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     1381 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     1441 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     1501 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     1561 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     1621 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     1681 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     1741 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     1801 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgtttttc
     1861 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     1921 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     1981 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     2041 agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     2101 aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca
     2161 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
     2221 ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc tgcagctgga
     2281 tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
     2341 caatgctttc ttataatgcc aactttgtac aagaaagctg ggtctgggca ggagacggag
     2401 cgtgcgatgt ctgggccact gcaggtgttg acgatcaggt ccagaggccc gaagaggaag
     2461 tgcacgagct ccgcggcgct ggg
//
