LOCUS       sequence-398141-        4299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC17A6_STOP
FEATURES             Location/Qualifiers
     source          1..4299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1856..1955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1972..1991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1973..1991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1996..2012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1996..2012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2114..2133)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2125..2934
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2192..2211)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3570..3589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3823..3840
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3999..4026)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4118..4204)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4253..4275
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4267..4284
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4268..4284
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgga gagcgtgaag
      121 cagaggatcc tggcacctgg caaggagggc ctgaagaact tcgccggcaa gtccctgggc
      181 cagatctaca gagtgctgga gaagaagcag gacacaggcg agaccatcga gctgacagag
      241 gatggcaagc cactggaggt gcccgagaga aaggcccccc tgtgcgactg tacctgcttt
      301 ggcctgcctc ggagatatat catcgccatc atgagcggcc tgggcttctg tatctccttt
      361 ggcatccggt gcaacctggg cgtggccatc gtggacatgg tgaacaatag caccatccac
      421 agaggcggca aagtgatcaa ggagaaggcc aagttcaatt gggatccaga gacagtgggc
      481 atgatccacg gctccttctt ttggggctac atcatcaccc agatccccgg cggctatatc
      541 gcctctaggc tggcagcaaa ccgcgtgttc ggagcagcaa tcctgctgac atctaccctg
      601 aatatgctga tcccaagcgc cgcaagggtg cactacggat gcgtgatctt tgtgagaatc
      661 ctgcagggcc tggtggaggg cgtgacatat cctgcctgcc acggaatctg gagcaagtgg
      721 gcaccacctc tggagcggtc cagactggcc accacaagct tctgtggatc ctacgcagga
      781 gccgtgatcg caatgccact ggccggcatc ctggtgcagt ataccggctg gagctccgtg
      841 ttctacgtgt atggcagctt tggcatggtg tggtacatgt tttggctgct ggtgtcctat
      901 gagtctcctg ccaagcaccc aacaatcacc gatgaggaga ggcgctacat cgaggagtct
      961 atcggagaga gcgccaacct gctgggagca atggagaagt tcaagacacc atggaggaag
     1021 ttctttacct ccatgcccgt gtatgccatc atcgtggcca atttctgtcg ctcttggacc
     1081 ttctacctgc tgctgatcag ccagcccgcc tattttgagg aggtgttcgg ctttgagatc
     1141 tctaaagtgg gcatgctgag cgccgtgcct cacctggtca tgaccatcat cgtgccaatc
     1201 ggcggccaga tcgccgactt cctgaggtcc aagcagatcc tgtctaccac aaccgtgcgc
     1261 aagatcatga actgcggcgg ctttggcatg gaggccacac tgctgctggt ggtgggatac
     1321 tcccacacca ggggagtggc catctctttc ctggtgctgg ccgtgggctt cagcggcttt
     1381 gccatctccg gctttaacgt gaatcacctg gatatcgccc ctcgctatgc ctccatcctg
     1441 atgggcatct ctaacggcgt gggcacactg tctggcatgg tgtgcccaat catcgtgggc
     1501 gccatgacca agaataagag ccgggaggag tggcagtacg tgttcctgat cgccgccctg
     1561 gtgcactacg gaggcgtgat cttctatgcc atctttgcat ccggagagaa gcagccatgg
     1621 gcagaccctg aggagacctc tgaggagaag tgcggcttta tccacgagga cgagctggat
     1681 gaggagacag gcgatatcac ccagaactac atcaattatg gcacaaccaa gagctacgga
     1741 gcaacaaccc aggcaaatgg aggatggcca tccggatggg agaagaagga ggagttcgtg
     1801 cagggcgagg tgcaggactc tcacagctac aaggaccggg tggattattc ctaagaccca
     1861 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1921 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1981 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2041 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2101 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2161 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2221 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2281 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2341 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2401 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2461 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2521 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2581 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2641 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2701 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2761 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2821 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     2881 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     2941 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3001 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3841 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3901 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3961 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4021 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4081 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4141 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4201 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4261 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
