LOCUS       sequence-398142-       11141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Boost the expression of pnRFP in mammalian cells (e.g., macrophage 
            RAW 264.7 cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Pang Y, Huang M, Fan Y, Yeh HW, Xiong Y, Ng HL, Ai HW
  TITLE     Development, Characterization, and Structural Analysis of a 
            Genetically Encoded Red Fluorescent Peroxynitrite Biosensor.
  JOURNAL   ACS Chem Biol. 2023 Jun 16;18(6):1388-1397. doi: 
            10.1021/acschembio.3c00139. Epub 2023 May 15.
  PUBMED    37185019
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.3c00139"; journalName: "ACS Chem Biol"; date: 
            "2023-06-16- 16"; volume: "18"; issue: "6"; pages: "1388-1397"
COMMENT     Sequence Label: pAcBAC3-POLY-pnRFP
FEATURES             Location/Qualifiers
     source          1..11141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..581
                     /label=chicken beta-actin promoter
     intron          582..1598
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1522..1544
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1606..1625
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1662..1671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     2393..2412
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2442..2459
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2491..3079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2544..2564)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2962..2973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3145..3164)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3210..3265
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3264..3283)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        3620..3868
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        3958..4052
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    4006..4024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4034..4052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     mobile_element  4158..4323
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4507..4961
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4594..4613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4803..4824
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4987..5091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5092..5160,5161..5952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5310..5329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6123..6711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6612..6631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6865..6882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  7016..7240
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(7307..7840)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(8029..8034,8035..8051,8052..8057))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(8560..8654)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    8560..8578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8588..8606
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(8744..8992)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        9118..9209
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     9134..9151
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(9268..9315,9316..10803)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    10930..11064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10967..10986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11021..11040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
      361 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
      421 gggggsgcgc gccaggsggg gsggggsggg gsgaggggsg gggsggggsg aggcggagag
      481 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
      541 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
      601 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
      661 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
      721 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
      781 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
      841 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
      901 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
      961 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     1021 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     1081 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     1141 cgggcggggg gtggcggcag gkgggggtgc cgggcggggc ggggccgcct cgggccgggg
     1201 rgggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     1261 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     1321 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     1381 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     1441 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     1501 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     1561 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     1621 ttgtgctgtc tcatcatttt ggcaaagaat tggccaagga ggccaccatg ggctcgagaa
     1681 taggtcggct gggctcaccc gtagtttccg agcggatgta ccccgaggac ggcgccctga
     1741 agagcgagat caagtagggg ctgaggctga aggacggcgg ccactacgcc gccgaggtca
     1801 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     1861 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtgc gaacgcgccg
     1921 agggccgcca ctccactggc ggcatggacg agctgtacaa gggaggtaca ggcgggagtc
     1981 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     2041 tgcacatgga gggctccgtg aacggccaca agttcgagat cgagggcgag ggcgagggcc
     2101 gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc cccctgccct
     2161 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac attaagcacc
     2221 cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg tgggagcgcg
     2281 tggtgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc ctgcaggacg
     2341 gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt cccccccgac ggccccgtaa
     2401 tgcagaagaa gaccatgggc tgggaggcta cgcgtgacca acaccatcac caccatcatt
     2461 aaatgcatga attcaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     2701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     2761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     2821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     2881 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     2941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3061 ctttgggccg cctccccgcg gcctggccgg ccgccagcac agtggtcgat cgaccaatgc
     3121 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     3181 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     3241 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     3301 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     3361 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3421 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3481 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3541 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     3601 gccctaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt tgcatatacg
     3661 atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa agatattagt
     3721 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     3781 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     3841 atatatcttg tggaaaggac gaaacaccgg tggggttccc gagcggccaa agggagcaga
     3901 ctctaaatct gccgtcacag acttcgaagg ttcgaatcct tcccccacca ttttttaata
     3961 tttgcatgtc gctatgtgtt ctgggaaatc accataaacg tgaaatccct atcagtgata
     4021 gagacttata agttccctat cagtgataga gacaccggag gggtagcgaa gtggctaaac
     4081 gcggcggact ctaaatccgc tccctttggg ttcggcggtt cgaatccgtc cccctccatt
     4141 ttttgctagg agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     4201 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     4261 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     4321 acagcggggc atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg
     4381 acaaaccgtc atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct
     4441 cttcgttatt aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg
     4501 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4561 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4621 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4681 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4741 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttaa
     4801 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4861 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4921 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6841 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     6901 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga ccagccgcgt
     6961 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg
     7021 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     7081 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     7141 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     7201 aaagaggggc gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac
     7261 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
     7321 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
     7381 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
     7441 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
     7501 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
     7561 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
     7621 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
     7681 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
     7741 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     7801 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     7861 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     7921 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     7981 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     8041 gaacaggctt atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg
     8101 gcaaccttgg gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag
     8161 gtttcggtct ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg
     8221 ctgtgcacgg atctgccctt gcttcaggag atcggtagac ctcggccgtc gcggcgcttg
     8281 ccggtggtgc tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat
     8341 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtac ccgtagtggc
     8401 tatggcaggg cttgcgctta atgcgccgct acagggcgcg tggggatacc ccctagagcc
     8461 ccagctgaaa aaatggaggg ggacggattc gaaccgccga acccaaaggg agcggattta
     8521 gagtccgccg cgtttagcca cttcgctacc cctccggtgt ctctatcact gatagggaac
     8581 ttataagtct ctatcactga tagggatttc acgtttatgg tgatttccca gaacacatag
     8641 cgacatgcaa atattaaaaa atggtggggg aaggattcga accttcgaag tctgtgacgg
     8701 cagatttaga gtctgctccc tttggccgct cgggaacccc accggtgttt cgtcctttcc
     8761 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
     8821 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
     8881 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
     8941 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcctgcc
     9001 cgacctagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
     9061 aaaacggatc ctgcaggtat ttgcggccgc ggtccgtata ctccggaata ttaatagatc
     9121 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     9181 acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg
     9241 ggcgcgaact cctaaaaaac cgccaccatg aagtgccttt tgtacttagc ctttttattc
     9301 attggggtga attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa
     9361 aatgttcctt ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac
     9421 ttaataggca cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac
     9481 ggttggatgt gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg
     9541 aagtatataa cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc
     9601 attgaacaaa cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga
     9661 tatgcaactg tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg
     9721 gttgatgaat acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat
     9781 tacatatgcc ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg
     9841 ctatgtgatt ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta
     9901 tcatccctgg gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga
     9961 ggcaaggcct gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc
    10021 tggttcgaga tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa
    10081 gggtcaagta tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt
    10141 gagaggatct tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt
    10201 ccaatctctc cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct
    10261 ttcaccataa tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt
    10321 gctgctccaa tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa
    10381 ctgtgggatg actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg
    10441 accagttcag gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat
    10501 cttcatctta gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg
    10561 caacttcctg atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc
    10621 gagcttgtag aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc
    10681 atagggttaa tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa
    10741 ttaaagcaca ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag
    10801 tgataagtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
    10861 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    10921 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    11041 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagttatta atagtaatca
    11101 attacggggt cattagttca tagcccatat atggagttcc g
//
