LOCUS       sequence-398156-        8089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Zhang J, Li Z, Pang Y, Fan Y, Ai HW
  TITLE     Genetically Encoded Boronolectin as a Specific Red Fluorescent 
            UDP-GlcNAc Biosensor.
  JOURNAL   ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409.
  PUBMED    37480329
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2023 Jul 22. doi: 10.1021/acssensors.3c00409."
COMMENT     Sequence Label: pMAH-Golgi-bapaUGAc
FEATURES             Location/Qualifiers
     source          1..8089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        739..757
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     739..756
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     770..789
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1832..1851
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1971..1988)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1977..2201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2247..2675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2334..2353)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2544..2565
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2684..2704)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2689..3018
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2869..3004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2931..2950
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3067..4092
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3067..4092
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4222..4343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4259..4278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4313..4332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4392..4408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4392..4408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4405..4427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4416..4432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4440..4446,4447..4464,4465..4470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4485..4506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4623..4640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4794..5382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4874..4893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5553..6344,6345..6413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6176..6195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6414..6518)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(6642..6732)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(6844..7092)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     promoter        complement(7381..7471)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(7583..7831)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      601 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
      661 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
      721 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
      781 tccacaggtg tccactccca ggtccaactg cacggaagct tgccaccatg aggcttcggg
      841 agccgctcct gagcggcagc gccgcgatgc caggcgcgtc cctacagcgg gcctgccgcc
      901 tgctcgtggc cgtctgcgct ctgcaccttg gcgtcaccct cgtttactac ctggctggcc
      961 gcgacctgag ccgcctgccc caactggtcg gagtctccac accgctgcag ggcggctcga
     1021 acagtgccgc cgccatcggg cagtcctccg gggagctccg gaccggaggg gccgaattcg
     1081 attcttattt tggaaaaact tataatccat gggatcctat gcagcggacc gtgtacatct
     1141 ccgagtccga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagtaggggc
     1201 tgaggctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     1261 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     1321 acgaggacta caccatcgtg gaacagtgcg aacgcgccga gggccgccac tccactggcg
     1381 gcatggacga gctgtacaag ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg
     1441 ataacatggc catcatcaag gagttcatga gcttcaaggt gcacatggag ggctccgtga
     1501 acggccacaa gttcgagatc gagggcgagg gcgagggccg cccctacgag gcctttcaga
     1561 ccgctaagct gaaggtgacc atcggtggcc ccctgccctt cgcctgggac atcctgtccc
     1621 ctcagttcat gtacggctcc aaggcctaca ttaagcaccc agccgacatc cccgactact
     1681 tcaagctgtc cttccccgag ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg
     1741 gcattattca cgttaaccag gactcctccc tgcaggacgg cgtattcatc tacaaggtga
     1801 agctgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct
     1861 gggaggctac gcctacgcct cgggtggtgg gtgatttata taagaatggt attgatccat
     1921 cttatactcc tcatcgtgac caatagggcc cgtttaaacc cgctgatcag cctcgactgt
     1981 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     2041 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     2101 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     2161 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     2221 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     2281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2641 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     2701 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2761 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     2821 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     2881 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2941 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3001 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     3061 cacgtgatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     3121 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     3181 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     3241 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     3301 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     3361 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     3421 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     3481 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     3541 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     3601 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     3661 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     3721 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     3781 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     3841 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     3901 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     3961 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     4021 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     4081 gcaaaggaat agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4141 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4201 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     4261 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4321 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     4381 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4441 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4501 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4561 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4621 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4681 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4741 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4801 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4861 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4921 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4981 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5041 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5101 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5161 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5221 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5281 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5341 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5401 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5461 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5521 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5581 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5641 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5701 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5761 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5821 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5881 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5941 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6001 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6061 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6121 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6181 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6241 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6301 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6361 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6421 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6481 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg actcgactcg
     6541 ctagtgttcc aaaaaatggt gggggacgga ttcgaaccgc cgaacccaaa gggagcggat
     6601 ttagagtccg cggcgtttgg ccacttcgct accccaccag agtggtctca tacagaactt
     6661 ataagattcc caaatccaaa gacatttcac gtttatggtg atttcccaga acacatagcg
     6721 acatgcaaat atgaattgat gactgcctga tggagaaaaa atggtggggg acggattcga
     6781 accgccgaac ccaaagggag cggatttaga gtccgcggcg tttggccact tcgctacccc
     6841 accggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat actttcaagt
     6901 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
     6961 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
     7021 aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
     7081 aaataggccc tcttcctgcc cgaccttcgc gatgtacggg ccagatatac gccaatattg
     7141 gccattagcc atattattca ttggttatat agcataaatc aatattggct attggccatt
     7201 gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc caatatgacc
     7261 gccatgttgg ctcgactcgc tagtgttcca aaaaatggtg ggggacggat tcgaaccgcc
     7321 gaacccaaag ggagcggatt tagagtccgc ggcgtttggc cacttcgcta ccccaccaga
     7381 gtggtctcat acagaactta taagattccc aaatccaaag acatttcacg tttatggtga
     7441 tttcccagaa cacatagcga catgcaaata tgaattgatg actgcctgat ggagaaaaaa
     7501 tggtggggga cggattcgaa ccgccgaacc caaagggagc ggatttagag tccgcggcgt
     7561 ttggccactt cgctacccca ccggtgtttc gtcctttcca caagatatat aaagccaaga
     7621 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
     7681 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     7741 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
     7801 aatatgaagg aatcatggga aataggccct cttcctgccc gaccttcgcg atgtacgggc
     7861 cagatatacg ccaatattgg ccattagcca tattattcat tggttatata gcataaatca
     7921 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7981 gctcatgtcc aatatgaccg ccatgttggc tcgagcattg attattgact agttattaat
     8041 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccg
//
