LOCUS       sequence-398157-        4851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  boronic acid- and peptide-assisted UDP-GlcNAc sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Zhang J, Li Z, Pang Y, Fan Y, Ai HW
  TITLE     Genetically Encoded Boronolectin as a Specific Red Fluorescent 
            UDP-GlcNAc Biosensor.
  JOURNAL   ACS Sens. 2023 Jul 22. doi: 10.1021/acssensors.3c00409.
  PUBMED    37480329
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2023 Jul 22. doi: 10.1021/acssensors.3c00409."
COMMENT     Sequence Label: pBad-bapaUGAc
FEATURES             Location/Qualifiers
     source          1..4851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     1083..1102
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1195..1212
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1252..1269)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1422..1508
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1600..1627
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1646..1737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1738..1806,1807..2598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1956..1975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2769..3357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3258..3277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3511..3528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3543..3683
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3609..3628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3769..3791
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3947..4825)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggctcgaga gattcttatt ttggaaaaac ttataatcca
      361 tgggatccta tgcagcggac cgtgtacatc tccgagtccg agcggatgta ccccgaggac
      421 ggcgccctga agagcgagat caagtagggg ctgaggctga aggacggcgg ccactacgcc
      481 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
      541 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtgc
      601 gaacgcgccg agggccgcca ctccactggc ggcatggacg agctgtacaa gggaggtaca
      661 ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      721 agcttcaagg tgcacatgga gggctccgtg aacggccaca agttcgagat cgagggcgag
      781 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac catcggtggc
      841 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      901 attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg
      961 tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc
     1021 ctgcaggacg gcgtattcat ctacaaggtg aagctgcgcg gcaccaactt cccccccgac
     1081 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcta cgcctacgcc tcgggtggtg
     1141 ggtgatttat ataagaatgg tattgatcca tcttatactc ctcatcgtga ccaacaccat
     1201 caccaccatc attaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     1261 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     1321 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     1381 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     1441 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1501 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     1561 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     1621 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
     1681 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1741 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1801 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1861 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1921 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1981 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2041 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2101 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2161 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2221 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2281 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2341 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2401 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2461 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2521 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2581 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2641 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2701 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2761 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2821 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2881 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2941 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3001 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3061 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3121 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3181 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3241 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3301 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3361 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3421 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3481 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3541 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     3601 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     3661 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     3721 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     3781 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
     3841 cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
     3901 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
     3961 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     4021 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     4081 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     4141 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     4201 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     4261 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     4321 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     4381 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     4441 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     4501 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     4561 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     4621 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     4681 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     4741 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     4801 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga g
//
