LOCUS       sequence-39817-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             58..291
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             484..495
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atattcattt ctttgcnagt tataaatact gaataataag atgacatgaa ctactactgc
       61 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
      121 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
      181 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
      241 tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct
      301 tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta
      361 ccgagctcga attccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
      421 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
      481 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
      541 acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg aattgtcagt
      601 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
      661 ggcaataggg agggggaaag cgaaagtc
//
