LOCUS       sequence-398170-       11717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant expression vector (2x35S) for direct cloning of amiRNAs into 
            Arabidopsis thaliana MIR390a precursor including only the basal 
            stem.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11717)
  AUTHORS   Cisneros AE, Martin-Garcia T, Primc A, Kuziuta W, Sanchez-Vicente J,
            Aragones V, Daros JA, Carbonell A
  TITLE     Transgene-free, virus-based gene silencing in plants by artificial 
            microRNAs derived from minimal precursors.
  JOURNAL   Nucleic Acids Res. 2023 Oct 27;51(19):10719-10736. doi: 
            10.1093/nar/gkad747.
  PUBMED    37713607
REFERENCE   2  (bases 1 to 11717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-10-27- 27"; volume: "51"; issue: 
            "19"; pages: "10719-10736"
COMMENT     Sequence Label: pMDC32B-BS-AtMIR390a-B/c
FEATURES             Location/Qualifiers
     source          1..11717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             443..1237
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1324..1912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1813..1832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2066..2083
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2098..2238
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2164..2183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2324..2346
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2666..2860
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2926..3999)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4428..5057)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6358..6382
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6570..6592
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6584..6601
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6585..6601
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7027..7371
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7431..7455
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(7517..7522,7523..7540,7541..7547)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7601..8260
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7667..7686)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8602..8907
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8786..8805
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8988..9012)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9076..9328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9345..9361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9345..9361)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9358..9380)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9369..9385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9393..9399,9400..9417,9418..9423))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9438..9459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9650..10326
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10393..11418
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    11459..11633
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    join(11711..11717,1..18)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
       61 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
      121 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
      181 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
      241 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
      301 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
      361 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
      421 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
      481 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
      541 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
      601 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
      661 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
      721 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
      781 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
      841 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
      901 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
      961 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     1021 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     1081 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     1141 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     1201 aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat
     1261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1441 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     1501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1561 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1621 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1681 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1741 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1801 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1861 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1921 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1981 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     2041 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     2101 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     2161 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     2221 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     2281 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     2341 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     2401 gcggtcgagt ggcgacggcg cggcttgtcc gcgcccccgt catcaccgaa acgcgcgagg
     2461 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     2521 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     2581 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     2641 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     2701 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     2761 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     2821 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagaa cttttcgacc
     2881 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     2941 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     3001 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     3061 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     3121 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     3181 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     3241 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     3301 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     3361 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     3421 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     3481 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     3541 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     3601 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     3661 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     3721 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     3781 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     3841 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     3901 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     3961 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     4021 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
     4081 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     4141 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     4201 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     4261 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     4321 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     4381 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     4441 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     4501 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     4561 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     4621 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     4681 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     4741 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     4801 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     4861 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     4921 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     4981 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     5041 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     5101 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     5161 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     5221 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     5281 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     5341 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     5401 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     5461 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     5521 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     5581 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     5641 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     5701 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
     5761 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
     5821 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
     5881 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
     5941 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
     6001 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
     6061 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
     6121 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
     6181 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
     6241 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
     6301 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
     6361 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
     6421 ctgatccaag ctcaagctgc tctagcattc gccattcagg ctgcgcaact gttgggaagg
     6481 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6541 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     6601 tgccaagctt gcatgcctgc aggtcaacat ggtggagcac gacacacttg tctactccaa
     6661 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     6721 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     6781 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     6841 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     6901 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
     6961 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
     7021 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
     7081 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
     7141 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
     7201 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     7261 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     7321 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct
     7381 agaggatccc cgggtaccgg gccccccctc gaggcgcgcc aagctatcaa acaagtttgt
     7441 acaaaaaagc aggctccgcg gccgccccct tcaccagtag agaagaatct gtaagagacc
     7501 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     7561 ttaggagccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     7621 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     7681 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     7741 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     7801 gaatgctcat ccggagttcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     7861 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     7921 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     7981 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     8041 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     8101 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     8161 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     8221 attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggag ccggcttact
     8281 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     8341 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     8401 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     8461 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     8521 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     8581 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     8641 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccggccg acggatggtg
     8701 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     8761 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtt
     8821 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     8881 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     8941 cctcgacggt ctcacattgg ctcttcttac taagggtggg cgcgccgacc cagctttctt
     9001 gtacaaagtg gttcgataat tcttaattaa ctagttctag agcggccgcc accgcggtgg
     9061 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     9121 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     9181 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     9241 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     9301 cgcgcggtgt catctatgtt actagatcgg gaattcgtaa tcatgtcata gctgtttcct
     9361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9541 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
     9601 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
     9661 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
     9721 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
     9781 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
     9841 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
     9901 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     9961 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
    10021 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
    10081 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
    10141 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
    10201 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
    10261 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
    10321 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagctttcg cagatccggg
    10381 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
    10441 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
    10501 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
    10561 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
    10621 gtgcttgaca ttggggagtt tagcgagagc ctgacctatt gcatctcccg ccgttcacag
    10681 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctaca accggtcgcg
    10741 gaggctatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
    10801 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
    10861 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
    10921 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
    10981 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
    11041 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
    11101 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
    11161 gcaggatcgc cacgactccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
    11221 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
    11281 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
    11341 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
    11401 ccgagggcaa agaaatagag tagatgccga ccgggatctg tcgatcgaca agctcgagtt
    11461 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
    11521 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
    11581 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa
    11641 ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
    11701 gcgtcaattt gtttaca
//
