LOCUS       sequence-398176-       10122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Andreu N, Zelmer A, Fletcher T, Elkington PT, Ward TH, Ripoll J, 
            Parish T, Bancroft GJ, Schaible U, Robertson BD, Wiles S
  TITLE     Optimisation of bioluminescent reporters for use with mycobacteria.
  JOURNAL   PLoS One. 2010 May 24;5(5):e10777.
  PUBMED    20520722
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-05-24"; volume: "5(5)"; pages: "e10777"
COMMENT     Sequence Label: pMV306G13+Lux
FEATURES             Location/Qualifiers
     source          1..10122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1915..1934)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1926..2741
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1999..2018)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3072..3660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3561..3580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4374..5456
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGVTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             5471..6454
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAAADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             6478..7920
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7933..8856
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVALRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             8881..9993
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQAELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      10054..10100
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
      661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
      721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
      781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
      841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
      901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
      961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgtg
     1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agccacctga
     1801 cgtcgggggg gggggaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
     1861 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     1921 gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
     1981 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2041 caatctatcg cttgtatggg aagccccatg cgccagagtt gtttctgaaa catggcaaag
     2101 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2161 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2221 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2281 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2341 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2401 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2461 ggaaagaaat gcataatctt ttgccattct caccggattc agtcgtcact catggtgatt
     2521 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     2581 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2641 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2701 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     2761 tgtaacactg gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg
     2821 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     2881 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcaccaaccg
     2941 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
     3001 accttcttca cgaggcagac ctcactagtt ccatgagcgt cagaccccgt agaaaagatc
     3061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3481 cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3781 gataccgctc gccgcagccg aacgaccgag cgcaacgcgt gcggccgcga tcgccactag
     3841 cgccgcggtc ggaatcagcg cactggccgc cggagcggtc agctacctgt tgctgcgccg
     3901 cttcgctttc tagcaccgac gtcccggcgc cagcagctac ctctcgagca aacgttttgc
     3961 gcgcccgaaa aataagtcgt tgcagaactt tcatgaatta ggccttgctg cgcccaggct
     4021 ccagtagtag aaatggagtc acggcagccc ggtgaagcca aggtcgaacc ggaagagaag
     4081 gttcgtcctc ccgacccggg cacccagcac ggcccccgga acccacgcgg agtcatagcc
     4141 gcgataatgg cagaagtgtt gcgggcctgc gtaattgcga aattcagatg gtgccgacgg
     4201 ccctttgggt ggggctgcag ccagaagggt cgcaaaagcg ccgaggccaa cccacgcagc
     4261 ccacaaatgc acgcttggta accgagaatt cgaatggcca tgggacgtcg acctgaggta
     4321 attataaccc gggccctata tatggatcca attcgtcgac aggaggactc tctatgaaat
     4381 ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac
     4441 gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg
     4501 agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac
     4561 ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc
     4621 cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt
     4681 ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata
     4741 acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcgtg acagagggat
     4801 atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt
     4861 atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg
     4921 ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag
     4981 cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg
     5041 accattgctt atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt
     5101 gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg
     5161 attcagacca aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa
     5221 aaggacataa agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa
     5281 cgccgcagga atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata
     5341 tttgttgtgg atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct
     5401 tccagtctga tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa
     5461 ggagaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca
     5521 agaacaaagt atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga
     5581 acagatttta gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac
     5641 tgtttctggt tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat
     5701 cattacaact catcatcctg tccgcatagc ggaggaagct tgcttattgg atcagttaag
     5761 tgaagggaga tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt
     5821 taatcgcccg gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga
     5881 tgctttaaca acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc
     5941 tgtaaatccc catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca
     6001 tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc
     6061 taatgatgtt agatatgaat atgctgaaag atataaagcc gctgcggata aatatgacgt
     6121 tgacctatca gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa
     6181 taaagctaaa caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa
     6241 tgaaaatttc gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac
     6301 ggagtgtata actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct
     6361 gtcctttgaa ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga
     6421 taatattaag aagtaccaca tgaaatatac ctaataggta ccaggaggaa ggcaaatatg
     6481 actaaaaaaa tttcattcat tattaacggc caggttgaaa tctttcccga aggtgatgat
     6541 ttagtgcaat ccattaattt tggtgataat agtgtttacc tgccaatatt gaatgactct
     6601 catgtaaaaa acattattga ttgtaatgga aataacgaat tacggttgca taacattgtc
     6661 aattttctct atacggtagg gcaaagatgg aaaaatgaag aatactcaag acgcaggaca
     6721 tacattcgtg acttaaaaaa atatatggga tattcagaag aaatggctaa gctagaggcc
     6781 aattggatat ctatgatttt atgttctaaa ggcggccttt atgatgttgt agaaaatgaa
     6841 cttggttctc gccatatcat ggatgaatgg ctacctcagg atgaaagtta tgttcgggct
     6901 tttccgaaag gtaaatctgt acatctgttg gcaggtaatg ttccattatc tgggatcatg
     6961 tctatattac gcgcaatttt aactaagaat cagtgtatta taaaaacatc gtcaaccgat
     7021 ccttttaccg ctaatgcatt agcgttaagt tttattgatg tagaccctaa tcatccgata
     7081 acgcgctctt tatctgttat atattggccc caccaaggtg atacatcact cgcaaaagaa
     7141 attatgcaac atgcggatgt tattgtcgct tggggagggc cagatgcgat taattgggcg
     7201 gtagagcatg cgccatctta tgctgatgtg attaaatttg gttctaaaaa gagtctttgc
     7261 attatcgata atcctgttga tttgacgtcc gcagcgacag gtgcggctca tgatgtttgt
     7321 ttttacgatc agcgagcttg tttttctgcc caaaacatat attacatggg aaatcattat
     7381 gaggaattta agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccgaat
     7441 gccaaaaaag attttgatga aaaggcggcc tattctttag ttcaaaaaga aagcttgttt
     7501 gctggattaa aagtagaggt ggatattcat caacgttgga tgattattga gtcaaatgca
     7561 ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc ttcatcacgt cgataatatt
     7621 gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa ccatatctat ttttccttgg
     7681 gagtcatcat ttaaatatcg agatgcgtta gcattaaaag gtgcggaaag gattgtagaa
     7741 gcaggaatga ataacatatt tcgagttggt ggatctcatg acggaatgag accgttgcaa
     7801 cgattagtga catatatttc tcatgaaagg ccatctaact atacggctaa ggatgttgcg
     7861 gttgaaatag aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccataa
     7921 taggtaaaaa gtatggaaaa tgaatcaaaa tataaaacca tcgaccacgt tatttgtgtt
     7981 gaaggaaata agaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga
     8041 aagaatgcca ttattattgc gtctggtttt gcccgcagga tggatcattt tgctggtctg
     8101 gcggaatatt tatcgcggaa tggatttcat gtgatccgct atgattcgct tcaccacgtt
     8161 ggattgagtt cagggacaat tgatgaattt acaatgtcta taggaaagca gagcttgtta
     8221 gcagtggttg attggttaac tacacgaaaa ataaataact tcggtatgtt ggcttcaagc
     8281 ttatctgcgc ggatagctta tgcaagccta tctgaaatca atgcttcgtt tttaatcacc
     8341 gcagtcggtg ttgttaactt aagatattct cttgaaagag ctttagggtt tgattatctc
     8401 agtctaccca ttaatgaatt gccgaataat ctagattttg aaggccataa attgggtgct
     8461 gaagtctttg cgagagattg tcttgatttt ggttgggaag atttagcttc tacaattaat
     8521 aacatgatgt atcttgatat accgtttatt gcttttactg caaataacga taattgggtc
     8581 aagcaagatg aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat
     8641 tctttgttag gaagttcgca tgacttgagt gaaaatttag tggccctgcg caatttttat
     8701 caatcggtta cgaaagccgc tatcgcgatg gataatgatc atctggatat tgatgttgat
     8761 attactgaac cgtcatttga acatttaact attgcgacag tcaatgaacg ccgaatgaga
     8821 attgagattg aaaatcaagc aatttctctg tcttaaagga tcctgaggag gaaaacaggt
     8881 atgacttcat atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt
     8941 ttttcgagcg atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt
     9001 gtgcttgatg catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag
     9061 gcacacaaag tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg
     9121 gtttttaagt ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc
     9181 agtagcggca cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga
     9241 ctcttaggct ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata
     9301 gaattagtca atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt
     9361 atgagtttgg tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat
     9421 tttgttaaaa cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt
     9481 attggttcgc catactttat ttatttactc tgccattata tgaaagataa aaaaatctca
     9541 ttttctggag ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa
     9601 gaatctctga aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat
     9661 attagtcaga tccgagatat atttaatcaa gctgaactca acacttgttt ctttgaggat
     9721 gaaatgcagc gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg
     9781 ttgaaacctg tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc
     9841 agttatccag catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag
     9901 tatcccggcg tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt
     9961 gctttaagct taaccgaagc gtttgatagt tgatatccat caagcttatc gatgtcgacg
    10021 tagttaacta gcgtacgatc gactgccagg catcaaataa aacgaaaggc tcagtcgaaa
    10081 gactgggcct ttcgttttat gccatcatgg ccgcggtgat ca
//
