LOCUS       sequence-39818-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pRRL.EF1a.HA.NLS.Sce(opt).T2A.BFP
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    167..288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(178..197)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     232..251
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             360..593
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
       61 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      121 gcgggatggg cggagttagg ggcgggatag ctagagccag acatgataag atacattgat
      181 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      241 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttcc tctcactctc
      301 tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg aactactact
      361 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
      421 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
      481 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
      541 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
      601 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg
      661 taccgagctc gaattccagg cggggaggcg gcccaaa
//
