LOCUS       sequence-398187-        1193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of recombinant BMPS K319E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Medoh UN, Hims A, Chen JY, Ghoochani A, Nyame K, Dong W, 
            Abu-Remaileh M
  TITLE     The Batten disease gene product CLN5 is the lysosomal 
            bis(monoacylglycero)phosphate synthase.
  JOURNAL   Science. 2023 Sep 15;381(6663):1182-1189. doi: 
            10.1126/science.adg9288.
  PUBMED    37708259
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2023-09-15- 15"; volume: "381"; 
            issue: "6663"; pages: "1182-1189"
COMMENT     Sequence Label: pRK5 BMPS K319E-6xHis
FEATURES             Location/Qualifiers
     source          1..1193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1133..1150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcccaggtcc aactgcacct cggttctatc gattgaattc accatggccc aggaggtcga
       61 cacagcacag ggagcagaga tgagacgggg cgccggggcc gccagaggac gggcctcctg
      121 gtgttgggca ctcgccctcc tgtggctggc tgtcgtgcct gggtggagca gagtttcagg
      181 catcccatct cgccgacact ggccagttcc ttataagaga tttgacttca gaccaaagcc
      241 cgacccgtat tgccaggcca agtacacatt ttgccctact gggtcaccca tacccgtcat
      301 ggagggagac gatgacatcg aagtgtttag attgcaagcc cccgtgtggg agttcaagta
      361 cggtgacctg cttggccatc tgaaaatcat gcatgacgca ataggtttcc ggtctaccct
      421 cacaggaaag aattacacca tggagtggta tgaactcttc cagctgggca attgcacatt
      481 cccacatctg agacctgaga tggatgcacc attctggtgt aaccagggcg ctgcctgctt
      541 cttcgaaggg atcgacgacg tgcattggaa agagaatggc accctggtac aggtcgcgac
      601 aattagcggc aatatgttta accagatggc caaatgggtg aagcaggaca acgagacagg
      661 catatactac gaaacatgga acgtgaaggc atcacctgag aagggcgctg aaacttggtt
      721 cgacagttat gattgcagca aatttgtcct gagaactttt aataagctgg cagagttcgg
      781 agccgaattc aaaaatattg aaaccaatta caccaggatc tttctttatt ccggcgagcc
      841 cacttacctg ggcaacgaaa cctcagtgtt cggtcctacc gggaataaga cgctcggttt
      901 ggccatcaaa cgcttctact accctttcaa gccacatttg cccaccaagg agttcctcct
      961 gagtctcctg cagatttttg atgctgtgat cgtccatgaa cagttttact tgttttacaa
     1021 tttcgaatat tggttcctcc ccatgaaatt ccctttcatc aagatcacat acgaggagat
     1081 ccccctcccc attaggaaca agactctgtc aggcctcggg ggcggcggaa gccaccatca
     1141 ccaccatcac taagcggccg ctaagtaagt aaggatcccc agcttggccg cca
//
