LOCUS       sequence-398205-       10199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For cytosine base editing using miniSdd3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Huang J, Lin Q, Fei H, He Z, Xu H, Li Y, Qu K, Han P, Gao Q, Li B, 
            Liu G, Zhang L, Hu J, Zhang R, Zuo E, Luo Y, Ran Y, Qiu JL, Zhao KT,
            Gao C
  TITLE     Discovery of deaminase functions by structure-based protein 
            clustering.
  JOURNAL   Cell. 2023 Jul 20;186(15):3182-3195.e14. doi: 
            10.1016/j.cell.2023.05.041. Epub 2023 Jun 27.
  PUBMED    37379837
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.05.041"; journalName: "Cell"; date: 
            "2023-07-20- 20"; volume: "186"; issue: "15"; pages: "3182-3195.e14"
COMMENT     Sequence Label: p2T-CMV-miniSdd3-BE4max-BlastR
FEATURES             Location/Qualifiers
     source          1..10199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(842..865,866..868,869..4942)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             842..4942
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4973..5221
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5252..5500
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5543..5563
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5572..5589
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5612..5629)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5618..5842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5913..5929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5913..5929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5926..5948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5937..5953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5961..5967,5968..5985,5986..5991))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6006..6027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6144..6265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6155..6174)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6209..6228
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6423..6821)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6840..6887)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6935..7264)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6949..7084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7003..7022)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7249..7269
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(7388..7575,7576..7587)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(7648..8236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7728..7747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8407..9198,9199..9267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9030..9049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9268..9372)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9451..9470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9662..9673,9674..9811)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        9820..10199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtccctaga agatttaacc cagcctgctg
      361 gcagggcctg aagccatatg accaagcagg cacagctgag ggcaacctct tcatcgcccc
      421 tgggaagcgc tggaataccc ggcccatgca ggccagcaaa ctggaggtgg gaccccagag
      481 tgacctgcac ccacagtgga gaagcaggaa ggctccctgg cacatcgaag gaaaaatcgc
      541 tgcctacatg aggcagaagg gcttcacaga tggctgtgtg tatctgaacg ccagaccctg
      601 ctctggccct gatggctgcg caagaaacct gcctgacctc ctgcctgtgg gtagcactct
      661 ccatgtccac gccaggtaca tcgacagaac aggagagaca agattctact accgtgaata
      721 ccgtgggacc ggaaaggcct tgacctccgg aggatctagc ggaggctcct ctggctctga
      781 gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc
      841 agacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat
      901 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      961 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     1021 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     1081 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     1141 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     1201 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     1261 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     1321 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     1381 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     1441 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     1501 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     1561 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     1621 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     1681 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     1741 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     1801 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     1861 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     1921 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     1981 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     2041 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     2101 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     2161 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     2221 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     2281 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     2341 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     2401 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     2461 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     2521 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     2581 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     2641 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     2701 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     2761 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     2821 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     2881 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     2941 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     3001 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     3061 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     3121 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     3181 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     3241 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     3301 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     3361 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     3421 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     3481 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     3541 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     3601 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     3661 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     3721 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     3781 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     3841 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     3901 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     3961 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     4021 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     4081 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     4141 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     4201 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     4261 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     4321 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     4381 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     4441 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     4501 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     4561 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     4621 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     4681 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     4741 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     4801 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     4861 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     4921 cctgtctcag ctgggaggtg acagcggcgg gagcggcggg agcgggggga gcactaatct
     4981 gagcgacatc attgagaagg agactgggaa acagctggtc attcaggagt ccatcctgat
     5041 gctgcctgag gaggtggagg aagtgatcgg caacaagcca gagtctgaca tcctggtgca
     5101 caccgcctac gacgagtcca cagatgagaa tgtgatgctg ctgacctctg acgcccccga
     5161 gtataagcct tgggccctgg tcatccagga ttctaacggc gagaataaga tcaagatgct
     5221 gagcggagga tccggaggat ctggaggcag caccaacctg tctgacatca tcgagaagga
     5281 gacaggcaag cagctggtca tccaggagag catcctgatg ctgcccgaag aagtcgaaga
     5341 agtgatcgga aacaagcctg agagcgatat cctggtccat accgcctacg acgagagtac
     5401 cgacgaaaat gtgatgctgc tgacatccga cgccccagag tataagccct gggctctggt
     5461 catccaggat tccaacggag agaacaaaat caaaatgctg tctggcggct caaaaagaac
     5521 cgccgacggc agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg tcatcatcac
     5581 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5641 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     5701 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     5761 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     5821 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     5881 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5941 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     6001 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6061 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6121 cgtattgggc gctacagaca tgataagata cattgatgag tttggacaaa ccacaactag
     6181 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     6241 cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc
     6301 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     6361 aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc ctgctcctcg gccacgaagt
     6421 gcttagccct cccacacata accagagggc agcaattcac gaatcccaac tgccgtcggc
     6481 tgtccatcac tgtccttcac tatggctttg atcccaggat gcagatcgag aagcacctgt
     6541 cggcaccgtc cgcaggggct caagatgccc ctgttctcat ttccgatcgc gacgatacaa
     6601 gtcaggttgc cagctgccgc agcagcagca gtgcccagca ccacgagttc tgcacaaggt
     6661 cccccagtaa aatgatatac attgacacca gtgaagatgc ggccgtcgct agagagagct
     6721 gcgctggcga cgctgtagtc ttcagagatg gggatgctgt tgattgtagc cgttgctctt
     6781 tcaatgaggg tggattcttc ttgagacaaa ggcttggcca tggtttagtt cctcaccttg
     6841 tcgtattata ctatgccgat atactatgcc gatgattaat tgtcaacacg tgctgatcag
     6901 atccgaaaat ggatatacaa gctcccggga gctttttgca aaagcctagg cctccaaaaa
     6961 agcctcctca ctacttctgg aatagctcag aggcagaggc ggcctcggcc tctgcataaa
     7021 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
     7081 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
     7141 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
     7201 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
     7261 acacattcca cagaattcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7381 aacatgtaag tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag
     7441 taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt
     7501 aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta
     7561 aaattactca agtactttac acctctgcat gtgagcaaaa ggccagcaaa aggccaggaa
     7621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca ctcagtggaa
     8281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8401 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8461 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8521 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     8581 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8641 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8701 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8761 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8821 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8881 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8941 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9001 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9061 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9121 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9181 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9241 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9301 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9361 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     9421 atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag
     9481 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9541 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     9601 ttttgcgctg cttcgcgatg tacgggccag atatacgcgg gtaccggcat atggttcttg
     9661 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
     9721 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
     9781 aagtactcca ttaaaattgt acttgagtat taacgcgttg acattgatta ttgactagtt
     9841 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     9901 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     9961 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10021 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    10081 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10141 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
