LOCUS       sequence-39821-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..751)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 angnatcttt aaaattttgt aatccagagg ttgattgtcg actctagaat caattaagct
       61 tgtgccccag tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga
      121 cgtaggtctc gttgttggcc tccttgattc tttccagtct gtagtccaca tagtagacgc
      181 caggcatctt gaggttctta gcgggtttct tggatctata tgtggtcttg atgtttgcga
      241 tcagatggct cccgcccacg agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt
      301 cagcggggta cagcgtctcg gtgaaggcct cccagccgag tgttttcttc tgcatcacag
      361 ggccgttgga tgtgaagttc acccctctga tcttgacgtt gtagatgagg cagccgtcct
      421 ggaggctggt gtcctgggta gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct
      481 cccatgtgaa gccctcaggg aaggactgct tgaagaagtc ggggatgccc tgggtgtggt
      541 tgatgaaggt cttgctgccg tagaggaagc tagtagccag gatgtcgaag gcgaagggga
      601 gagggccgcc ctcgaccacc ttgattctca tggtctgggt gccctcgtag ggcttgcctt
      661 cgccctcgga tgtgcacttg aagtgatggt tgtccacggt gccctccatg tacagcttca
      721 tgtgcatgtt ctccttaatc agctcgctca tggggcccgg attctcctcc a
//
