LOCUS       sequence-39822-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..181
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             369..380
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcaatggtt
       61 acaaataaag caatagcatc acaaatttca caaataaaca tatggttaca aataaagcaa
      121 tagcatcaca aatttcacaa ataaattata acttcgtata atgtatgcta tacgaagtta
      181 tgatatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
      241 aggtaccgag ctcgaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga
      301 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
      361 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
      421 gcaacacagg cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg
      481 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
      541 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
      601 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
      661 acaacgggcc acaactcctc ataaagagac agcaaccagg att
//
