LOCUS       sequence-398229-        2858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Ctdnep1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Ctdnep1
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    198..297
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(901..1000)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1112..1131)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1123..1932
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1190..1209)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2075..2663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2564..2583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2798..2825)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
      181 gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      241 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctcca
      301 tgctgccctt atctcctttg tcccggaatc gcctagccca ggtcaaaaga aaaattctcg
      361 tgctggatct ggacgaaacc ctgatccatt cgcatcatga cggggtgctg aggcctacag
      421 tgagacctgg gacacctccc gacttcatcc tcaaggtcgt gattgataag catccagtcc
      481 ggttttttgt acataagagg ccccatgtgg atttcttctt agaagtggta agccagtggt
      541 atgagcttgt ggtgttcaca gcaagcatgg aaatttatgg ctctgctgtg gcagataaac
      601 tggacaacag cagaagcatt cttaagagga gatactacag acagcactgc actttggagt
      661 tgggcagcta catcaaagac ctctccgtgg tccacagcga cctgtccagc atcgtgatcc
      721 tggacaactc ccccggggct tacaggagcc acccagacaa tgccatcccc atcaaatcct
      781 ggttcagtga ccccagtgac acagcccttc tcaaccttct cccaatgctg gatgccctca
      841 ggttcactgc tgatgtccga tcggtgctga gccgaaacct tcaccaacat aggctctggg
      901 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
      961 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
     1021 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
     1081 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     1141 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1201 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1261 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1321 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1381 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1441 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1501 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1561 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1621 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1681 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     1741 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     1801 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     1861 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     1921 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     1981 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2041 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2101 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2161 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2221 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2281 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2341 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2401 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2461 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2521 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2581 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2641 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2701 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2761 gtattaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     2821 cttctgctta gtttgatgcc tggcagttta tggcgggc
//
