LOCUS       sequence-398236-        2618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_181-299.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_181-299
FEATURES             Location/Qualifiers
     source          1..2618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(59..145)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    198..297
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(661..760)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(872..891)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             883..1692
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(950..969)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1835..2423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2324..2343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2558..2585)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
       61 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      121 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
      181 gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      241 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctcca
      301 tgcggccgca gaccctgaag ggacaccagg agaagattcg gcagcggcag tccatcctgc
      361 ctcctcccca gggcccggcg cccatcccct tccagcaccg cggcggggat tccccggagg
      421 ccaagaatcg cgtgggcccg caggtgccac tcagcgagcc aggtttccgc cgtcgggagt
      481 cgcaggagga gccgcgggcc gtgctggctc agaagataga gaaggagacg caaatcctca
      541 actgcgccct ggacgacatc gagtggtttg tggcccggct gcagaaggca gccgaggctt
      601 tcaagcagct gaaccagcgg aaaaagggga agaagaaggg caagaaggcg ccagcagagg
      661 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
      721 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg
      781 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca
      841 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
      901 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
      961 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1021 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1081 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     1141 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     1201 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1261 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     1321 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1381 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1441 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     1501 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     1561 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     1621 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     1681 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     1741 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1801 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1861 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1921 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1981 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2041 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2101 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2161 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2221 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2281 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2341 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2401 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2461 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2521 gtattaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     2581 cttctgctta gtttgatgcc tggcagttta tggcgggc
//
