LOCUS       sequence-398240-        2777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_544-715.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_544-715
FEATURES             Location/Qualifiers
     source          1..2777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(104..203)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      256..342
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      434..461
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(596..1184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(676..695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1327..2136)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2050..2069
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2128..2147
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2259..2358
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggtagcttgt gggtcgggct ggcggggccc cagtacttct gaccggcctg ctcgaacggg
       61 gcgccggcgt cctccggtcg cgcctcgcct aggatgttca tggagcctgc ttttttgtac
      121 aaagttggca ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca ggtcactatc
      181 agtcaaaata aaatcattat ttggggcccg agatccatgc tagcgttaac gcgagagtag
      241 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
      301 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
      361 aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
      421 catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
      481 cctggctagc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1261 gcttgcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa
     1321 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     1381 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     1441 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     1501 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     1561 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     1621 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     1681 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     1741 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     1801 aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg tgagtaacca
     1861 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     1921 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     1981 cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg cacctgattg
     2041 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     2101 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2161 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2221 gagattttga gacacgggcc agagctgcag ctggatggca aataatgatt ttattttgac
     2281 tgatagtgac ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt
     2341 tgtacaagaa agctgggtcg ctgtcctccc ccctcctctg attcatggaa tgaaacttgt
     2401 tcatgagttc ttccagctcc gacccacttt gctgcttctc caggaaggcc ttctgcatgg
     2461 tgagctggct gtacacgcgg acgccctcct cgccgcacac tttcttcagc tcctccttgt
     2521 tgagggagaa gagctgcggc ccggtcagga tgcccaggtt ctccacgatc cgcgggctga
     2581 aggccttggc ttccagccag gcgcggacct cgtccggacc cgactcgtag gtgagcggct
     2641 ggctcacggg ctggctgcgc tccacgcgga agtgcctctg tggctgcgcc cttatattcg
     2701 atatcttgcg gatgagctcg tcgttgacct cgtccatgtg ctgcatgagt tcgtccttat
     2761 tgcccgggaa gcttggg
//
