LOCUS       sequence-398241-        2810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_1-183.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_1-183
FEATURES             Location/Qualifiers
     source          1..2810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(131..230)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      283..369
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      461..488
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(623..1211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(703..722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1354..2163)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2077..2096
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2155..2174
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2286..2385
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gagttggaat acttcttcct ctgctcaaac aggtccttgg ggctcatctt ggccacaccg
       61 tcggaccggc ccaggctgcc attggtggca cccgggcagc agctcacggc cccggactgg
      121 ctcatcatgg agcctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca
      181 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgaga
      241 tccatgctag cgttaacgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      301 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      361 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      421 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      481 ggcctttttg cgtttctaca aactcttcct ggctagcggt aatacggtta tccacagaat
      541 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      601 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      661 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      721 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1021 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1261 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct
     1321 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     1381 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     1441 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     1501 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     1561 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
     1621 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     1681 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     1741 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     1801 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     1861 tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     1921 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     1981 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     2041 caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     2101 taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct
     2161 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     2221 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga gctgcagctg
     2281 gatggcaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata
     2341 agcaatgctt tcttataatg ccaactttgt acaagaaagc tgggtcctgc ggccgcatct
     2401 tcttgcccag ccggcagtcg gccaacgcgc tctcgatgtc ctcgtgcacc agctctgcct
     2461 ccacctcatc gcagtggaag aagtggacat ccggcttgct ctgctccgag tcctggcaca
     2521 cgagcagcag cacagacggg tagcgcagct ggttgaggac cgtctggctg cgctgcaccg
     2581 tgggcagcgg gaagtcttcc agctcctcct gtgactcgat gtccagcagc cgcagcgact
     2641 ggtcgttcac ctgcagcagc atctcctggg tccagatctt ctccttggag ctcagctgca
     2701 ccagcttccg gatggcgtcg tccacagacg tgatggcttc gctcttgtcc atgatgaatg
     2761 tggccaggtg ctggacgtgg tactgcgagg tctcgtgcat gatgacgttg
//
