LOCUS       sequence-398243-        2651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for Gateway with Eps8L2_367-496.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, 
            Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER
  TITLE     Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear 
            positioning during cell migration.
  JOURNAL   Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 
            10.1016/j.cub.2021.01.007. Epub 2021 Feb 9.
  PUBMED    33567288
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.01.007"; journalName: "Curr Biol"; date: 
            "2021-04-12- 12"; volume: "31"; issue: "7"; pages: "1521-1530.e8"
COMMENT     Sequence Label: pDONR201-Eps8L2_367-496
FEATURES             Location/Qualifiers
     source          1..2651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(65..151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    204..303
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(700..799)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(911..930)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             922..1731
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(989..1008)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1874..2462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2363..2382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2597..2624)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
       61 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      121 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      181 acgctagcat ggatctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt
      241 cgttgcaaca aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagcag
      301 gctccatggt ctcctgccca ctgctctccc gagatgccgt ggacttcctg cgcggccacc
      361 tggtccctaa ggagatgtcg ctgtgggagt cactgggaga gagctggatg cggccccgtt
      421 ccgagtggcc gcgggagcca caggtgcccc tctacgtgcc caagttccac agcggctggg
      481 agcctcctgt ggatgtgctg caggaggccc cctgggaggt ggaggggctg gcgtctgccc
      541 ccatcgagga ggtgagtcca gtgtcgaggc aaagtatccg gaactcccag aagcacagcc
      601 ccacttcaga gcccaccccc ccgggggatg ccctaccacc agtcagctcc ccacatactc
      661 acaggggcta ccagccaaca ccagccatgg ccaaatatga cccagctttc ttgtacaaag
      721 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      781 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
      841 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
      901 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
      961 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1021 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1081 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1141 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1201 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1261 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1321 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     1381 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     1441 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     1501 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     1561 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     1621 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     1681 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     1741 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     1801 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1981 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2521 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgct agccaggaag
     2581 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     2641 ggcagtttat g
//
