LOCUS       sequence-398279-       12100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Hong C, Moorefield KS, Jun P, Aldape KD, Kharbanda S, Phillips HS, 
            Costello JF
  TITLE     Epigenome scans and cancer genome sequencing converge on WNK2, a 
            kinase-independent suppressor of cell growth.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Jun 26. 104(26):10974-9.
  PUBMED    17578925
REFERENCE   2  (bases 1 to 12100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Jun 26. 104(26):10974-9."
COMMENT     Sequence Label: WNK2 WT
FEATURES             Location/Qualifiers
     source          1..12100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        520..723
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     768..787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        768..786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      816..825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7503..7544
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7554..7571
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7594..7611)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7600..7824
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7870..8298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7957..7976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8167..8188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8307..8327)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8312..8641
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8492..8627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8708..9502
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8762..8781)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9372..9391
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9678..9799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9715..9734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9769..9788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9848..9864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9848..9864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9861..9883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9872..9888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9896..9902,9903..9920,9921..9926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9941..9962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10079..10096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10250..10838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10330..10349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11009..11800,11801..11869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11632..11651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11870..11974)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12049..12068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
       61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      721 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
      781 tatagggaga cccaagctgg ctagttaagc ttgccgccac catggacggc gatggcggcc
      841 gccgagacgt ccccggcacg ctgatggagc ccgggcgcgg cgcggggccc gcgggcatgg
      901 cggagcctcg ggcgaaggcg gcgcggccgg ggccccagcg ctttctgcgg cgcagcgtgg
      961 tagagtcgga ccaggaggag ccgccgggct tggaggcagc cgaggcgccg ggcccgcagc
     1021 ccccgcagcc cctgcagcgc cgggtgcttc tgctctgcaa gacgcgccgc ctcatcgcgg
     1081 agcgcgctcg cggacgcccc gccgcccccg cgcccgcagc gctggtagcg cagccgggag
     1141 cccccggagc ccccgcggac gccggccccg agcccgtggg cacgcaggag cccggcccgg
     1201 accccatcgc agccgctgtc gaaaccgcgc ctgcccccga cggcggcccc agggaggagg
     1261 cggcggcgac cgtgaggaag gaggatgagg gggcggccga ggcgaagcct gagcccgggc
     1321 gcactcgccg ggacgagccc gaagaggagg aggacgacga ggacgacctc aaggccgtgg
     1381 ccacctctct ggacggccgc ttcctcaagt tcgacatcga gctgggccgc ggttccttca
     1441 agacggtcta caaggggctg gacacggaga cctgggtgga ggtggcctgg tgtgagctgc
     1501 aggaccggaa gctcaccaag ctggagcggc agcggttcaa ggaagaggct gagatgctga
     1561 aaggcctgca gcaccccaac atcgtgcgct tctacgactt ctgggagtcc agcgccaagg
     1621 gcaagcggtg cattgtgctg gtgacggagc tgatgacctc agggacgctg aagacatacc
     1681 tgaagcggtt caaggtgatg aagcccaagg ttctccgcag ctggtgccgg cagatcctga
     1741 agggcctgct gttcctgcac acaaggacgc cacccatcat ccaccgagac ctgaaatgtg
     1801 acaatatttt catcaccgga ccaactgggt ccgtgaagat tggcgacttg ggcctggcca
     1861 ctctgaaaag agcgtcattt gccaaaagtg tgataggtac tcccgagttc atggcgcccg
     1921 agatgtacga ggagcactac gatgagtccg tggacgtcta tgcctttggg atgtgcatgc
     1981 tggagatggc cacctcggag tacccctact cggagtgcca gaatgcggcc cagatctacc
     2041 gcaaggtcac ctgtggtatc aagccggcca gctttgagaa agtgcacgat cctgaaatca
     2101 aggagattat tggggagtgt atctgcaaaa acaaggagga aaggtacgag atcaaagacc
     2161 tgctgagcca cgccttcttc gcagaggaca caggcgtgag ggtggagctc gcggaggagg
     2221 accacggcag gaagtccacc atcgccctga ggctctgggt ggaagacccc aagaaactga
     2281 agggaaagcc caaggacaat ggagccatag agttcacctt cgacctggag aaggagacgc
     2341 cggatgaggt ggcccaagag atgattgagt ctggattctt ccacgagagt gacgtcaaga
     2401 tcgtggccaa gtccatccgt gaccgcgtgg ccttgatcca gtggcggcgg gagaggatct
     2461 ggcccgcgct gcagcccaag gagcagcagg atgtgggcag cccggacaag gccaggggtc
     2521 cgccggtgcc cctgcaggtc caggtgacct accatgcaca ggctgggcag cccgggccac
     2581 cagagcccga ggagccggag gccgaccagc acctcctgcc acctacgttg ccgaccagcg
     2641 ccacctccct ggcctcggac agcaccttcg acagcggcca gggctctacc gtgtactcag
     2701 actcgcagag cagccagcag agcgtgatgc ttggctccct tgccgacgca gcgccgtccc
     2761 cggcccagtg tgtgtgcagc ccccctgtga gcgaggggcc cgtcctgccg cagagcctgc
     2821 cctcgctggg ggcctaccag cagcccacgg ctgcacctgg cttgccggtg ggctctgtcc
     2881 cggcccccgc ctgccctccg tccctccagc agcacttccc ggatccggcc atgagcttcg
     2941 cccccgtgct gccgccgccc agcaccccca tgcccacggg cccaggccag ccagcacccc
     3001 ccggccagca gcctcctccg ctggcccagc cgacacccct gccgcaggtc ctggccccac
     3061 agcccgtggt ccccctccag ccggttcccc cccacctgcc accgtacctg gctccagcct
     3121 cccaggtggg ggcccccgct cagctgaagc ccctccagat gccacaggcg cccctgcagc
     3181 cgcttgctca agtccctccg cagatgcccc cgattcctgt tgtgcccccc atcacgcccc
     3241 tggcgggaat cgacggcctc cctccggccc tcccagacct gccgaccgcg actgtgcctc
     3301 ccgtgccacc acctcagtat ttctctccag ccgtgatctt gccgagcctc gctgccccac
     3361 tcccccctgc gtccccagcc ttgcctctgc aggctgtgaa gctgccccac ccccctgggg
     3421 cgcccctggc catgccctgc cggaccattg tgccaaatgc accggccact atccccctgc
     3481 tggccgtagc cccaccgggc gtggctgccc tgtccattca ttctgccgtg gcccagctcc
     3541 caggccaacc tgtgtaccca gcggccttcc cacagatggc gcctactgac gtccctcctt
     3601 ccccccatca cacggtgcag aatatgaggg ccacccctcc acagccggca ctgcctccac
     3661 aacccacact gcccccacaa cccgtgctgc ccccgcaacc cacgctgccc cctcaacctg
     3721 tgttgccccc gcaacccaca cggccccctc aacctgtgct gcccccgcaa cccatgctgc
     3781 ccccacaacc tgtgctgccc ccgcagccgg cactgcctgt gcgccctgag cccctccagc
     3841 cccaccttcc tgaacaagct gctccagctg ctacaccagg gagccagatt ctgcttggcc
     3901 acccagctcc ctatgctgtg gacgtcgccg ctcaggtccc caccgtgcct gtgccaccgg
     3961 ctgcggtcct ctccccgcct ctgccggaag tgctgctgcc tgccgcccct gagctcctgc
     4021 ctcagttccc cagctccctg gccacggtgt ctgcctctgt gcagagtgtg cccacccaga
     4081 ctgccacact tctgccacca gcaaacccac cgctgcctgg cgggcccggg atcgccagcc
     4141 cttgcccaac tgtccagctg acggtggaac cagtccaaga ggagcaggcc tcacaggaca
     4201 agccgcccgg cctcccgcag agctgtgaga gctatggagg ttctgatgtc acttctggaa
     4261 aagagctgag tgacagctgt gaaggcgcct ttggaggggg caggctggag ggcagggcag
     4321 cccgaaaaca ccaccgcagg tccacgcgtg cgcgctcccg gcaggagagg gccagccggc
     4381 cccggcttac catcttgaac gtgtgcaaca ctggggacaa gatggtggag tgccagctgg
     4441 agacacacaa ccacaagatg gtgaccttca agttcgactt ggacggggac gcacccgatg
     4501 aaattgccac gtatatggtg gagcatgact ttatcctgca ggccgagcgg gaaacgttca
     4561 tcgagcagat gaaggatgtc atggacaagg cagaggacat gctcagcgag gacacagacg
     4621 ccgaccgtgg ctccgaccca gggaccagcc cgccacacct cagcacctgc ggcctgggca
     4681 ccggggagga gagccgacaa tcccaagcca acgcccccgt gtatcagcag aacgtcctgc
     4741 acaccgggaa gaggtggttc atcatctgtc cggtggctga gcaccccgcc cccgaggccc
     4801 ctgaatcttc gcccccactt cctctaagct ccctgccgcc agaagccagc caagattcag
     4861 cgccctataa agaccagctg tcctcgaagg aacaacccag ctttctagcc agtcagcagc
     4921 tcctgagcca ggcgggcccc agcaaccctc ctggggcacc cccagcccct ttggccccct
     4981 cctcccctcc tgtgactgct ctgccccaag atggagcagc tccagccacc agcaccatgc
     5041 cagagccagc gtcaggaact gccagccagg cagggggtcc agggacacct caggggctga
     5101 ccagtgagct cgagacgtct cagccactag cggagactca cgaggccccg cttgctgtgc
     5161 agcccctcgt ggtgggccta gcaccttgca ctccagctcc agaggctgcc tcaaccaggg
     5221 acgccagtgc cccaagggag cccctgccac ctcctgcacc tgagcccagc ccccacagcg
     5281 ggaccccaca gcccgccttg ggtcagcctg ctcccctgct tcctgccgca gtgggggccg
     5341 tcagcctggc cacctcccag ctcccaagcc cacccctggg gcccaccgtc cccccacagc
     5401 caccctcggc cctggagtcg gatggggaag ggccgccccc cagggtgggc tttgtggaca
     5461 gcaccatcaa gagcctggac gagaagctgc ggactctgct ctaccaggag cacgtgccca
     5521 cctcctcagc ctcagctggg acccctgtgg aggtgggcga cagagacttc accctggagc
     5581 ccctgagagg ggaccagccc cgctcagagg tctgcggggg ggacctggcc ctgcccccag
     5641 tgcctaagga ggcggtctca gggcgtgtcc agctgcccca gcccttggtg gagaagtcag
     5701 aactggcccc cactcgaggg gccgtgatgg agcagggcac gtcctcgtca atgacagcag
     5761 agtcgtctcc caggagtatg ctaggctatg acagagatgg aaggcaggtg gcctcagact
     5821 cccatgtggt ccccagcgtc ccccaggatg tacctgcttt tgtgagacct gcacgtgtgg
     5881 agcccacaga cagggatggt ggagaagctg gagaaagctc ggcagagccc ccgccgagtg
     5941 acatgggcac agtggggggc caggctagcc acccccagac actcggcgct cgagctttgg
     6001 ggtcccctcg gaaacgtcca gagcagcagg atgtcagctc accagccaag actgtgggcc
     6061 gtttctcggt ggtcagcact caggacgagt ggaccctggc ctccccccac agcctgagat
     6121 actctgcccc acccgacgtc tacctggacg aggccccctc cagccccgac gtgaagctgg
     6181 cagtgcggcg ggcgcagacg gcctcctcca tcgaggtcgg cgtgggcgag cccgtgtcca
     6241 gcgactctgg ggacgagggc cctcgggcga gacccccggt gcagaagcag gcgtccctgc
     6301 ccgtgagtgg cagcgtggct ggcgacttcg tgaagaaggc caccgccttc ctgcagaggc
     6361 cttctcgggc cggctcgctg ggccccgaga cacccagcag ggtgggcatg aaggtcccca
     6421 cgatcagcgt gacctccttc cattcccagt cgtcctacat cagcagcgac aatgattcgg
     6481 agctcgagga tgctgacata aagaaggagc tgcagagtct gcgggagaag cacctgaagg
     6541 agatctcgga gctgcagagc cagcagaagc aggagatcga agctctgtac cgccgcctgg
     6601 gcaagccact gccccccaac gtgggcttct tccacacggc accccccact ggccgccgga
     6661 gaaaaaccag caagagcaag ctgaaggcag gcaagctgct aaatcccctg gtgcggcagc
     6721 tcaaggtcgt ggcctccagc acaggtcact tggctgactc cagcagaggc cctcccgcta
     6781 aggaccctgc ccaagccagt gtggggctca ctgcagacag cacgggcctg agcgggaagg
     6841 cagtgcagac ccagcagccc tgctccgtcc gggcctccct gtcttcggac atctgctccg
     6901 gcttagccag tgatggaggc ggagcgcgtg gccaaggctg gacggtttac cacccaacgt
     6961 ctgagagagt gacctataag tctagtagca aacctcgtgc tcgattcctc agtggacccg
     7021 tatctgtgtc catctggtct gccctgaagc gtctctgcct aggcaaagaa cacagcagta
     7081 ggtcctccac cagcagcctg gccccaggcc ctgagccagg cccccagccc gccctgcacg
     7141 tccaggcgca ggtgaacaac agcaacaaca agaagggtac cttcacggac gacctgcaca
     7201 agctggtgga cgagtggacg agcaagacgg tgggggccgc gcagctgaag cccacgctca
     7261 accagctgaa gcagacccag aagctgcaag acatggaggc ccaggcaggc tgggctgccc
     7321 ctggcgaggc gcgggctatg accgcacctc gagcaggagt ggggatgcca cgtctgcccc
     7381 cagcgcccgg ccctctgtcc accacggtca ttcccggagc cgccccgacc ctgtccgtgc
     7441 ccacaccaga tcctgagagt gagaagcctg acagtgatca tctagagggc ccgcggttcg
     7501 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     7561 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     7621 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7681 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7741 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7801 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     7861 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7921 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7981 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     8041 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8101 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8161 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8221 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8281 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8401 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8461 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8521 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8581 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8641 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     8701 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     8761 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     8821 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     8881 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     8941 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     9001 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     9061 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     9121 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     9181 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     9241 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     9301 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     9361 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     9421 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     9481 ccttcttgac gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga
     9541 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     9601 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     9661 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9721 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9781 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     9841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    10561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    10621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    10681 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    10741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    10801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11041 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    11641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12001 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
    12061 tgatgccgca tagttaagcc agtatctgct ccctgcttgt
//
