LOCUS       sequence-398280-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a guide RNA targeting UBA6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer 
            SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster 
            JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root 
            DE, McFarland JM, Vazquez F, Hahn WC
  TITLE     A Ubiquitination Cascade Regulating the Integrated Stress Response 
            and Survival in Carcinomas.
  JOURNAL   Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 
            10.1158/2159-8290.CD-22-1230.
  PUBMED    36576405
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1158/2159-8290";
            journalName: "Cancer Discov"; date: "2023-03-1- 1"; volume: "13"; 
            issue: "3"; pages: "766-795"
COMMENT     Sequence Label: pXPR_003-sgUBA6
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     416..433
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    550..571
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(586..591,592..609,610..616)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    624..640
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     629..651
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     648..664
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     648..664
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     683..703
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        685..703
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        731..957
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             958..1138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1185..1310
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1803..2036
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2221..2265
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2443..2691
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2443..2463
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2614..2633
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2713..2788
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2844..2961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3100..4278
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3331..4269
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4226..4246
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4291..4890
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4291..4310)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4787..4807
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4906..5494
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4959..4979)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5377..5388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5566..5799
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5877..5998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5914..5933)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5968..5987
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6038..6173
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6193..6212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6194..6212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6222..6239)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6222..6238)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6231..6253)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6380..6835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6467..6486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6677..6698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6861..6965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6966..7034,7035..7826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7184..7203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7997..8323,1..262)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       61 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      421 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      481 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      541 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      601 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      661 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
      721 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
      781 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
      841 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
      901 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
      961 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1021 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1081 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1141 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1201 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1261 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1321 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1381 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1441 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1501 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1561 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1621 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1681 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1741 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1801 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1861 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1921 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1981 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2041 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2101 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2161 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2221 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2281 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2341 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2401 ttatcgtttc agacccacct cccaaccccg aggggaccca gagagggcct atttcccatg
     2461 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     2521 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     2581 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     2641 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgctggaacc
     2701 atatttacat gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     2761 acttgaaaaa gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga
     2821 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     2881 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     2941 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
     3001 cggctcgagg gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     3061 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     3121 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     3181 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3241 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3301 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3361 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3421 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3481 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3541 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3601 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3661 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3721 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3781 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3841 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3901 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3961 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     4021 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     4081 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     4141 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4201 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4261 ccatttcagg tgtcgtgacg tacggccacc atgaccgagt acaagcccac ggtgcgcctc
     4321 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4381 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     4441 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4501 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4561 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4621 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     4681 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     4741 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     4801 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     4861 tggtgcatga cccgcaagcc cggtgcctga acgcgttaag tcgacaatca acctctggat
     4921 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4981 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5041 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5101 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5161 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5221 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5281 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5341 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5401 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5461 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     5521 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     5581 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     5641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5761 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     5821 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     5881 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     5941 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6001 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6061 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6121 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     6181 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     6241 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     6301 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     6361 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6781 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     6841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     7021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     7081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     7141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg ggg
//
