LOCUS       sequence-39830-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Wall NR, Wickersham IR, Cetin A, De La Parra M, Callaway EM
  TITLE     Monosynaptic circuit tracing in vivo through Cre-dependent targeting
            and complementation of modified rabies virus.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21848-53. doi: 
            10.1073/pnas.1011756107
  PUBMED    21115815
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2010-12-14- 14"; 
            volume: "107"; issue: "50"; pages: "21848-53"
COMMENT     Sequence Label: pAAV-FLEX-hGTB
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    69..102
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    165..198
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccctgc agggccgcca
       61 ccatggccat aacttcgtat aaagtatcct atacgaagtt atatcaaaat aggaagacca
      121 atgcttcacc atcgacccga attgccaagc atcaccatcg acccataact tcgtataatg
      181 tatgctatac gaagttatac ttcaactagt tcacagtctg gtctcgccgc cgctcttgtg
      241 gctctcccag ctgctgatga tcttgccgct ctggggggtc acgctcacct ctctgccggt
      301 gcctctcagg ttgtgctggg tgggctcgct tctgttcact cttctgcagc aggtcatcag
      361 gaagatgatc agcatcaggg cggtcagggc gccggcgctc agcagcacgt acttgcccca
      421 gttgggcagg cccaggtcca cgccgctcac ctggttgtgc acgtcgggca ggtgcacctc
      481 cacgaagtcc tcggcctcgt cgccgtcctt gaacacggtg ctggggtcgg ccagggggtg
      541 caccaggggg atcacgctgc tctccagcag ctccatgtgc tgctgcagca ggctgctctg
      601 catctcgggg atcagcacgt tgccgtcggg gcccaggatg atgccgttga agaacacgcc
      661 gttcacgtgg gggtggcatc tgccgcccac tctcaggcag cccttgctgg gcaggatctc
      721 gttccaggtt ctcacgctct tgtagtgggc gtcggcctcc atcagggtct tgttgaagat
      781 ggtgtaggcc ttgccgaagc cgggcaccag ctttctcagg tggctcagtc ttctgaagct
      841 cacgctcttg gtggtcatga tgctctc
//
