LOCUS       sequence-39831-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Wall NR, Wickersham IR, Cetin A, De La Parra M, Callaway EM
  TITLE     Monosynaptic circuit tracing in vivo through Cre-dependent targeting
            and complementation of modified rabies virus.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21848-53. doi: 
            10.1073/pnas.1011756107
  PUBMED    21115815
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2010-12-14- 14"; 
            volume: "107"; issue: "50"; pages: "21848-53"
COMMENT     Sequence Label: pAAV-FLEX-hGTB
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..83
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             573..632
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 acgagctgta caagaagctt ggtagtggag agggcagagg aagtctgcta acatgcggtg
       61 acgtcgagga gaatcctggc ccaatcgatg cgcggctgct gcccgcgctg ctgctgctgc
      121 tgctgcccgg taacgtgacc ggtaacgggt ccggtaacgg ttctttgtcc cgttgccccc
      181 ccggtcagtt ccgctgctcg gagccgcccg gtgcccacgg ggagtgttac ccgcaggact
      241 ggctgtgcga cggacacccc gactgcgacg acgggcggga cgagtggggc tgcgggacca
      301 gcgcgatccc cgcggtgccc acggacaacg gcacagaggc tcccactgcc cctgctcctg
      361 gacgtgctct gccagccagg aatcacggcc gcatgtggat gctgatcact gcagtgctcc
      421 tgtgctgcct ggtagctgtg ggtggtatcg ctgcatgggg gaagtccaaa gcaaaaagca
      481 ggtctgacat cttcagtctt gaaagcgcat ccaaggagct gctggtgcct gacaagagcc
      541 aggcagactt gttctccgct agcggtagtg gtcaatgtac taactacgct ttgttgaaac
      601 tcgctggcga tgttgaaagt aaccccggtc cttccggagt gccccaggcc ctgctgttcg
      661 tgcccctgct ggtgttcccc ctgtgcttcg gcaagttccc catctacacc atccccgaca
      721 agctgggccc ctggagcccc atcgacatcc accacctgag ctgccccaac aacctggtg
//
