LOCUS       sequence-39841-e         539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: LCMV:ECFP(loxP)MCS
FEATURES             Location/Qualifiers
     source          1..539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    142..276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(179..198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     233..252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(278..311)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     protein_bind    complement(278..311)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          432..497
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtaatc tagaggatca taatcagcca taccacattt
       61 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
      121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
      181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      241 tccaaactca tcaatgtatc ttatcatgtc tggatccata acttcgtata gnatacatta
      301 tacgaagtta tacctaggag atctcaccgg tgactagtca ggggggatct gcatggatct
      361 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
      421 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
      481 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaat
//
