LOCUS       sequence-398429-       10592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vector for encoding an engineered glycosylase-based guanine base 
            editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide 
            RNA compatible with SpG driven by hU6, mCherry driven by CBh 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Tong H, Liu N, Wei Y, Zhou Y, Li Y, Wu D, Jin M, Cui S, Li H, Li G, 
            Zhou J, Yuan Y, Zhang H, Shi L, Yao X, Yang H
  TITLE     Programmable deaminase-free base editors for G-to-Y conversion by 
            engineered glycosylase.
  JOURNAL   Natl Sci Rev. 2023 May 16;10(8):nwad143. doi: 10.1093/nsr/nwad143. 
            eCollection 2023 Aug.
  PUBMED    37404457
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Natl Sci 
            Rev."; date: "2023-05-16"; volume: "10(8):nwad143. doi"; pages: " 
            10.1093/nsr/nwad143. eCollection 2023 Aug"
COMMENT     Sequence Label: gGBEv6.3-SpG
FEATURES             Location/Qualifiers
     source          1..10592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        144..392
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     144..164
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     315..334
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        411..486
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        573..1751
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          804..1742
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1699..1719
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1768..1787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1768..1786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1836..1856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1869..5969
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6015..6035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7011..7031
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7043..7177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7080..7099)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7134..7153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        7191..7476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        7478..7755
                     /label=chicken beta-actin promoter
     intron          7756..7983
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      8002..8011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8008..8718
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8153..8171)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    8741..8965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(8973..9561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9053..9072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9732..10523,10524..10592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEMGAS
                     LIKHW"
     primer_bind     10355..10374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gacgtctaag taggagggcc tatttcccat gattccttca tatttgcata
      181 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      241 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      301 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      361 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
      421 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      481 cggtgctttt ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta
      541 gcgcgtgcgc caattctgca gacaaatggc tcggctccgg tgcccgtcag tgggcagagc
      601 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      661 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      721 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      781 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      841 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      901 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      961 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     1021 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     1081 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     1141 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1201 agcgcacatg ttcggcgagg cggggcctgc gagcgcggca accgagaatc ggacgggggt
     1261 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1321 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1381 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1441 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1501 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1561 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1621 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1681 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1741 aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga gccgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtctctg
     1861 gcggctcaga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg
     1921 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     1981 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     2041 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     2101 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     2161 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     2221 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     2281 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     2341 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     2401 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     2461 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     2521 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     2581 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     2641 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     2701 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     2761 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     2821 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     2881 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     2941 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     3001 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
     3061 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     3121 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     3181 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     3241 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     3301 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     3361 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     3421 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     3481 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     3541 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     3601 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     3661 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     3721 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     3781 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     3841 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     3901 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     3961 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     4021 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     4081 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     4141 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     4201 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     4261 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     4321 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     4381 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     4441 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     4501 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     4561 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     4621 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     4681 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     4741 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     4801 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     4861 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     4921 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     4981 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     5041 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     5101 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     5161 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     5221 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcctt tggcccaccg
     5281 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     5341 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     5401 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     5461 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccaagc
     5521 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     5581 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     5641 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     5701 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     5761 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     5821 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagcaa tacagaagca
     5881 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     5941 ggatcgacct gtctcagctg ggaggtgact ctggcggctc aaagcggacc gccgacggct
     6001 ctgaattcga gagccctaag aagaaaagaa aggtgctggg aggtgacagc ggcgggagcg
     6061 gcgggagcgg ggggagcgtc acccccgctt tgcagatgaa gaaaccaaag cagttttgcc
     6121 gacggatggg gcaaaagaag cagcgaccag ctagagcagg gcagccacac agctcgtccg
     6181 acgcagccca ggcacctgca gagcagccac acagctcgtc cgatgcagcc caggcacctt
     6241 gccccaggga gcgctgcttg ggaccgccca ccactccggg cccataccgc agcatctatt
     6301 tctcaagccc aaagggccac cttacccgac tggggttgga gttcttcgac cagccggcag
     6361 tccccctggc ccgggcattt ctgggacagg tcctagtccg gcgacttcct aatggcacag
     6421 aactccgagg ccgcatcgtg gagaccgagg catacctggg gccagaggat gaagccgccc
     6481 actcaagggg tggccggcag accccccgca accgaggcat gttcatgaag ccggggaccc
     6541 tgtacgtgta catcatttac agaatgtact tctgcatggg catctccagc caggggagag
     6601 gggctaacgt cttgctgcga gcactggagc ccctggaagg tctggagacc atgcgtcagc
     6661 ttcgcgccac cctccgggcc gccaccgccg cccgtgtcct cgccgaccgc gagctctgca
     6721 gtggcccctc caagctgtgc caggccctgg ccatcaacaa gagctttgac cagagggacc
     6781 tggcacagga tgaagctgta tggctggagc gtggtcccct ggagcccagt gagccggctg
     6841 tagtggcagc agcccgggtg ggcgtcggcc atgcagggga gtgggcccgg aaacccctcc
     6901 gcttctatgt ccggggcagc ccctgggtca gtgtggtcga cagagtggct gagagagaca
     6961 cacaggcctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     7021 agaggaaagt ctaaacgcgt cgaacttgtt tattgcagct tataatggtt acaaataaag
     7081 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7141 gtccaaactc atcaatgtat cttatcatgt ctggatcggc ggccggtacc cgttacataa
     7201 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7261 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7321 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     7381 ggtaaatggc ccgcctggca ttgtgcccag tacatgacct tatgggactt tcctacttgg
     7441 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     7501 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     7561 ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg cggggcgggg
     7621 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
     7681 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
     7741 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
     7801 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
     7861 gacggccctt ctcctccggg ctgtaattag ctgagcaaga ggtaagggtt taagggatgg
     7921 ttggttggtg gggtattaat gtttaattac ctggagcacc tgcctgaaat cacttttttt
     7981 caggttggca gatccgctag cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     8041 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     8101 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     8161 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     8221 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     8281 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg atgacggcgg cgtggtgacc
     8341 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     8401 accaacttcc cctccgacgg ccccgtaatg cagaagaaaa ccatgggctg ggaggcctcc
     8461 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     8521 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     8581 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     8641 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     8701 gacgagctgt acaagtaaac cggtctcttg gcccggatcc ctgtgccttc tagttgccag
     8761 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8821 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8881 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaaaacaa tagcaggcat
     8941 gctggggatg cggtgggctc tatggtggaa gctttccata ggctccgccc ccctgacgag
     9001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9721 tggtctgaca gttaccaatg cttaatcagt gaggcaccca tctcagcgat ctgtctattt
     9781 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9841 ccatctggcc ccagtgctgc aatgataccg cggcttccac gctcaccggc tccagattta
     9901 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9961 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10021 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10081 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10141 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10201 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10261 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10321 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10381 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10441 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10501 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10561 ataagggcga cacggaaatg ttgaatactc at
//
