LOCUS       sequence-39843-e         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: LCMV:ECFP(loxP)(FRT)MCS
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    133..267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     224..243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(275..308)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    316..349
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          474..539
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ggacgagctg tacaagtaat ctagaggatc ataatcagcc ataccacatt tgtagaggtt
       61 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      241 atcaatgtat cttatcatgt ctggatctga attcataact tcgtatagca tacattatac
      301 gaagttatag gatccgaagt tcctattctc tagaaagtat aggaacttcc cgggcctagg
      361 agatctcacc ggtgactagt caggggggat ctgcatggat ctttgtgaag gaaccttact
      421 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
      481 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
      541 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgc
//
