LOCUS       sequence-398436-        3296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre recombinase staging plasmid to make transposon insertion 
            libraries..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Tague N, Andreani V, Fan Y, Timp W, Dunlop MJ
  TITLE     Comprehensive Screening of a Light-Inducible Split Cre Recombinase 
            with Domain Insertion Profiling.
  JOURNAL   ACS Synth Biol. 2023 Oct 3. doi: 10.1021/acssynbio.3c00328.
  PUBMED    37788288
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Oct 3. doi: 10.1021/acssynbio.3c00328."
COMMENT     Sequence Label: pATT-Dest-Cre
FEATURES             Location/Qualifiers
     source          1..3296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      159..747
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     648..667
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     901..918
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1017..1036
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1017..1035
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1067..1089
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2152..2170)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2166..2213
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2319..2423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2424..2492,2493..3284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2642..2661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
       61 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      121 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      181 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      241 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      301 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      361 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      421 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      481 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      541 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      601 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      661 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      721 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      781 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      841 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      901 agcgagtcag tgagcgagga agcggaagag cgccatcagc aaccgcacct gtggcgccgg
      961 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
     1021 acgactcact atagggacac cacaacggtt tccctctaga aataattttg tttaacttta
     1081 agaaggagat ataccatcgt ctctgtccgc cacctctgat gaagtcagga agaacctgat
     1141 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     1201 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
     1261 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
     1321 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
     1381 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
     1441 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
     1501 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
     1561 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
     1621 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     1681 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     1741 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     1801 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     1861 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     1921 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     1981 aatcatgcag gctggtggct ggaccaatgt gaacattgtg atgaactaca tcagaaacct
     2041 ggactctgag actggggcca tggtgaggct gctcgaggat ggggacagcg agagacggtt
     2101 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     2161 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     2221 gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcaagg
     2281 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2401 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2461 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2521 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2581 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2641 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
     2701 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2761 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2821 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2881 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2941 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
     3001 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3061 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3121 ctggagccgg tgagcgtggc tctcgcggta tcattgcagc actggggcca gatggtaagc
     3181 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3241 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaa
//
