LOCUS       sequence-398498-        3222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Odd assembly vector with sfGFP dropout for Odd level assemblies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGLDV_41 [11o1G]
FEATURES             Location/Qualifiers
     source          1..3222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     185..204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(423..527)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     626..648
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     640..657
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     641..657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             954..965
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             972..1685
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1933..1949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1933..1949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1946..1968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      2139..2527
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(2660..2684)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2784..3222,1..353,354..422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
       61 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
      121 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
      181 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
      241 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
      301 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
      361 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
      421 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
      481 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
      541 aaagtgccag atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
      601 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
      661 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacctt agcgtgcgaa
      721 cagcacgcta ttgttgtatc attactcgca tccattctca ggctgtctcg tctcgtctcc
      781 gagctcttca atgaggagtg agaccgaaag tgaaacgtga tttcatgcgt cattttgaac
      841 attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt
      901 cttaacatcg cggcaactca agaaacggca ggttcggatc ttagctacta gagaaagagg
      961 agaaatacta gatgcgtaaa ggcgaagaac tgttcactgg tgtcgtccct attctggtgg
     1021 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
     1081 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggttcctt
     1141 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
     1201 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
     1261 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
     1321 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac ggcaatatcc
     1381 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
     1441 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
     1501 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
     1561 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     1621 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     1681 acaaatgatt cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg
     1741 cggtggacag gatcggcggt tttcttttct cttctcaagg tctcacgcta gcatgaagag
     1801 caccccagga tacatagatt accacaactc cgagcccttc cacctagcgt gctaaccacg
     1861 cacgctattg ttgtataggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     1921 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     1981 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     2041 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2101 aaggccgcgt tgctggcgtt acagctgggc gcgccccatg tcagccgtta agtgttcctg
     2161 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     2221 tgttgtccac aaccgttaaa ccttaaaggc tttaaaagcc ttatatattc ttttttttct
     2281 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     2341 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     2401 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     2461 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     2521 tcagatcggc gcgccggccg gccgaagatc ctttgatctt ttctacgggg tctgacgctc
     2581 tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt
     2641 cctcgtgata ccaattcgga gcctgctttt ttgtacaaac ttgttgataa tggcaattca
     2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2761 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2821 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2881 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagagcc acgctcaccg
     2941 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3001 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3061 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3121 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3181 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ct
//
