LOCUS       sequence-398501-        3166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Odd assembly vector with sfGFP dropout for Odd level assemblies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGLDV_42 [21o1G]
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     296..315
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     380..399
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     509..525
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     509..525
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      526..572
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     686..708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     700..717
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     701..717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             1014..1025
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1032..1745
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1993..2009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1993..2009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2006..2028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      2199..2587
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(2739..3166,1..388))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(2739..3166,1..388))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
       61 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
      121 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
      181 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
      241 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
      301 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc
      361 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
      421 acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt
      481 gagacacaac gtggctttcc cccgccgctc tagaactagt ggatccaaat aaaacgaaag
      541 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcgcatta tacgagacgt
      601 ccaggttggg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
      661 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
      721 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacctt agcgtgcgaa
      781 cagcacgcta ttgttgtatc attactcgca tccattctca ggctgtctcg tctcgtctcc
      841 gagctcttca atgaggagtg agaccgaaag tgaaacgtga tttcatgcgt cattttgaac
      901 attttgtaaa tcttatttaa taatgtgtgc ggcaattcac atttaattta tgaatgtttt
      961 cttaacatcg cggcaactca agaaacggca ggttcggatc ttagctacta gagaaagagg
     1021 agaaatacta gatgcgtaaa ggcgaagaac tgttcactgg tgtcgtccct attctggtgg
     1081 aactggatgg tgatgtcaac ggtcataagt tttccgtgcg tggcgagggt gaaggtgacg
     1141 caactaatgg taaactgacg ctgaagttca tctgtactac tggtaaactg ccggttcctt
     1201 ggccgactct ggtaacgacg ctgacttatg gtgttcagtg ctttgctcgt tatccggacc
     1261 atatgaagca gcatgacttc ttcaagtccg ccatgccgga aggctatgtg caggaacgca
     1321 cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc ggaagtgaaa tttgaaggcg
     1381 ataccctggt aaaccgcatt gagctgaaag gcattgactt taaagaggac ggcaatatcc
     1441 tgggccataa gctggaatac aattttaaca gccacaatgt ttacatcacc gccgataaac
     1501 aaaaaaatgg cattaaagcg aattttaaaa ttcgccacaa cgtggaggat ggcagcgtgc
     1561 agctggctga tcactaccag caaaacactc caatcggtga tggtcctgtt ctgctgccag
     1621 acaatcacta tctgagcacg caaagcgttc tgtctaaaga tccgaacgag aaacgcgatc
     1681 atatggttct gctggagttc gtaaccgcag cgggcatcac gcatggtatg gatgaactgt
     1741 acaaatgatt cagccaaaaa acttaagacc gccggtcttg tccactacct tgcagtaatg
     1801 cggtggacag gatcggcggt tttcttttct cttctcaagg tctcacgcta gcatgaagag
     1861 caccccagga tacatagatt accacaactc cgagcccttc cacctagcgt gctaaccacg
     1921 cacgctattg ttgtataggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     1981 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     2041 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     2101 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2161 aaggccgcgt tgctggcgtt acagctgggc gcgccccatg tcagccgtta agtgttcctg
     2221 tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac atccctggct
     2281 tgttgtccac aaccgttaaa ccttaaaggc tttaaaagcc ttatatattc ttttttttct
     2341 tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat tttattgttc
     2401 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat gagagcttag
     2461 tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt aaacatgaga
     2521 gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa ctgctgatct
     2581 tcagatcggc gcgccggccg gccgaagatc ctttgatctt ttctacgggg tctgacgctc
     2641 tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt
     2701 ccgctctgcc agtgttacaa ccaattaaca aattctgatt agaaaaactc atcgagcatc
     2761 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     2821 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2881 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2941 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     3001 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     3061 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     3121 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaacc
//
