LOCUS       sequence-398541-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 plasmid, barcoded ORI with gentamicin resistance cassette 
            and mScarlet-I reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 936 to 970)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL2_168
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(9..14,15..31,32..37)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             227..760
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        936..970
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     oriT            1896..2004
                     /note="incP origin of transfer"
     primer_bind     complement(2158..2174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2158..2174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2171..2193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      2364..2752
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(2901..2995)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3016..3675)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3590..3609
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3676..3778)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3865..3887
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3879..3896
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3880..3896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4314..5165)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(5152..5991)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(6502..7473)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            8712..8799
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      9141..9535
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 gcaaggagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
       61 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      121 tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag
      181 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgaatgt tacgcagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg
      301 catcattcgc acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga
      361 tcttttcggt cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga
      421 ttacctcggg aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga
      481 agcggttgtt ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga
      541 gatctatatc tatgatctcg cagtctccgg agagcaccgg aggcagggca ttgccaccgc
      601 gctcatcaat ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca
      661 agcagattac ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga
      721 agtgatgcac tttgatatcg acccaagtac cgccacctaa gcttgagcgc atgctcgagt
      781 acttcgcctg gaccatgctc gccgtcgtct tcggcttcct cctcttcgtc aacctcgcct
      841 acgtccccct cggccactgg gccgaggcct tcgccggctt cttcaaattt tcagggctgc
      901 cgcaccccat cgactggggg ctcgctatac aggagttgac ggctagctca gtcctaggta
      961 cagtgctagc tactaggagg aaaaaaatgg tgagtaaggg tgaagccgtg attaaagagt
     1021 tcatgcgctt caaagtgcat atggagggct caatgaacgg ccatgaattt gaaattgaag
     1081 gtgaaggaga aggccgtccg tatgagggca cccagaccgc caaactgaaa gtcacaaagg
     1141 gtggcccgct gccgttttcg tgggatattt tatcccctca attcatgtac ggcagccggg
     1201 cgtttattaa acacccggcg gatatcccgg attactataa gcaatctttt ccagaaggct
     1261 ttaaatggga gcgcgtgatg aattttgaag acggaggtgc tgttaccgtg acccaggaca
     1321 cgagcttaga agacggtacc ttgatctata aggtaaagct gcgtggtacc aatttcccgc
     1381 cggatgggcc agttatgcag aaaaagacta tgggctggga agcatcgacg gagcgtcttt
     1441 accccgaaga cggcgtcctg aaaggcgata ttaaaatggc cctgcgactg aaagacggcg
     1501 gtcgctacct tgcagatttc aaaacaacgt ataaagcgaa aaaacctgtg cagatgcccg
     1561 gggcgtacaa cgttgatcgc aaactagata tcacttcaca caacgaagat tacacggtag
     1621 tcgaacagta tgaacgtagc gaggggcgcc atagcaccgg aggcatggat gaactctata
     1681 aataagcttc gctcgtgctc gccgcgacct tcctcttcat gcacctcttc ggcatcgggc
     1741 tgcacaacgc tacagaggag cttttccgct gcataaccct gcttcggggt cattatagcg
     1801 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
     1861 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
     1921 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
     1981 aatcctgctc tgcgaggctg gccgtacgct aggtatactt gagaccaccc caggatacat
     2041 agattaccac aactccgagc ccttccacct agcgtgctaa ccacgcacgc tattgttgta
     2101 taggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
     2161 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
     2221 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
     2281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2341 gcgttacagc tgggcgcgcc ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg
     2401 ctttgagagg ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg
     2461 ttaaacctta aaggctttaa aagccttata tattcttttt tttcttataa aacttaaaac
     2521 cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta
     2581 gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag
     2641 ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa
     2701 catgagagct tagtacgtac tatcaacagg ttgaactgct gatcttcaga tcggcgcgcc
     2761 ggccggccga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     2821 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtccccc catacgatat
     2881 aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     2941 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3001 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     3061 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     3121 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     3181 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     3241 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     3301 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     3361 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     3421 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     3481 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     3541 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     3601 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     3661 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     3721 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     3781 gtctcattga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg
     3841 atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg
     3901 cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctta gcgtgcgaac
     3961 agcacgctat tgttgtatca ttactcgcat ccattctcag gctgtctcgt ctcgtctccg
     4021 aggtctcagg agaatgagtc agggtcacgc gtaggacgcg tcgagtaggg tgacttgtgg
     4081 ccctttcttt tgttccgcag ccacacgccg tgtgaagctg gggagataca tctggaagca
     4141 aagccaggaa agcggcctat ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat
     4201 attgttaagc cttttctgag catggtattt ttcatggtat taccaattag caggaaaata
     4261 agccattgaa tataaaagat aaaaatgtct tgtttacaat agagtggggg gggtcagcct
     4321 gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc
     4381 agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa
     4441 ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt
     4501 ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc
     4561 agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc
     4621 gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc
     4681 gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa
     4741 aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg
     4801 atttcctttg ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc
     4861 tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg
     4921 ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca
     4981 tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac
     5041 gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg
     5101 gccagacagt gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc
     5161 accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc
     5221 gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac
     5281 accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc
     5341 gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc
     5401 ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg
     5461 caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg
     5521 ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac
     5581 catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat
     5641 gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc
     5701 ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc
     5761 ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg
     5821 cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg
     5881 cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg
     5941 cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct
     6001 cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct
     6061 ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg
     6121 cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca
     6181 gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag
     6241 aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag
     6301 gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata
     6361 accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga
     6421 agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag
     6481 gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg
     6541 ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc
     6601 catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc
     6661 cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct
     6721 ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac
     6781 cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg
     6841 ccgctcgggc agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg
     6901 ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt
     6961 ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg
     7021 gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc
     7081 gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc
     7141 caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg
     7201 gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc
     7261 atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt
     7321 gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc
     7381 ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg
     7441 gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag ccagatcgag
     7501 ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat cagcagcacc
     7561 accgtaggca tcatggaagc cagcatcacg gttagccata gcttccagtg ccacccccgc
     7621 gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg cggctacctc ccgcaactct
     7681 ttggccagct ccacccatgc cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc
     7741 tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc
     7801 gccatgctct gggccagcgg ttcgatctgc tccgctaact cgttgatgcc tctggatttc
     7861 ttcactctgt cgattgcgtt catggtctat tgcctcccgg tattcctgta agtcgatgat
     7921 ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg
     7981 gccttccatc tccaccacgt tcggccccag gtgaacaccg ggcaggcgct cgatgccctg
     8041 cgcctcaagt gttctgtggt caatgcgggc gtcgtggcca gcccgctcta atgcccggtt
     8101 ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat gccttgggct tgagcgcttc
     8161 ggtcttctgt gccccgccct tctccggggt cttgccgttg taccgcttga accactgagc
     8221 ggcgggccgc tcgatgccgt cattgatccg ctcggagatc atcaggtggc agtgcgggtt
     8281 ctcgccgcca ccggcatgga tggccagcgt atacggcagg cgctcggcac cggtcaggtg
     8341 ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg gtcagctcga ccggcagggc
     8401 aaattcgacc tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg cagcatccca
     8461 gtagtcggcg ggccgctcga cgaactccgg catgtgcccg gattcggcgt gcaagacttc
     8521 atccatgtcg cgggcatact tgccttcgcg ctggatgtag tcggccttgg ccctggccga
     8581 ttggccgccc gacctgctgc cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg
     8641 ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg
     8701 tggccgttag gccagtttct cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag
     8761 ggtcgggatt gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg
     8821 ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa
     8881 caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag
     8941 cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac
     9001 agcagcgagt ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac
     9061 cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc
     9121 gaccgagaca ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg
     9181 ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc
     9241 gggctttgcc cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag
     9301 cgaatagacc agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca
     9361 caagggcgct gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc
     9421 caacaccccg ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt
     9481 gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca
     9541 gttagtacgg gagtgacggg cgcta
//
