LOCUS       sequence-39870-e       10474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Chandler RL, Chandler KJ, McFarland KA, Mortlock DP
  TITLE     Bmp2 transcription in osteoblast progenitors is regulated by a 
            distant 3' enhancer located 156.3 kilobases from the promoter.
  JOURNAL   Mol Cell Biol. 2007 Apr;27(8):2934-51. Epub 2007 Feb 5.
  PUBMED    17283059
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-04"; volume: "27(8)"; pages: "2934-51. Epub 2007
            Feb 5"
COMMENT     Sequence Label: pIBG-FTet
FEATURES             Location/Qualifiers
     source          1..10474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(728..1302,1303..1305,1306..1314)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(895..912)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1122..1141
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1327..4368
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             4393..5196
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4456..4475)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5066..5085
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5502..5636
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5539..5558)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5593..5612
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5689..5722
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(5999..6625)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     6530..6549
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        6641..6696
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    6647..6665
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    6677..6695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             6704..7909
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     protein_bind    8174..8207
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8286..8306)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8286..8304)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8325..8341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8325..8341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8338..8360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8349..8365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8373..8379,8380..8397,8398..8403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8418..8439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8556..8573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8727..9315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8807..8826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9486..10277,10278..10346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10109..10128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10347..10451)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccctgctag cttaattaac tcgaggtcga gatctggact ctagagaatt ccggcccccc
      721 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      781 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      841 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      901 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      961 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     1021 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     1081 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     1141 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     1201 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     1261 accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac aaccatggaa
     1321 gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1441 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
     1501 gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc
     1561 ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc
     1621 attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca
     1681 tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt
     1741 aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt
     1801 ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg
     1861 atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc
     1921 ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat
     1981 gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg
     2041 tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag
     2101 gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc
     2161 gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg
     2221 aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa
     2281 gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc
     2341 aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc
     2401 atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc
     2461 gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc
     2521 ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg
     2581 accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc
     2641 gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct
     2701 aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat
     2761 gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg
     2821 gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta
     2881 cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt
     2941 ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc
     3001 gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg
     3061 gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg
     3121 gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt
     3181 ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat
     3241 agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt
     3301 gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg
     3361 cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc
     3421 gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc
     3481 agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat
     3541 ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca
     3601 cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt
     3661 gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg
     3721 gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg
     3781 gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg
     3841 aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt
     3901 accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc
     3961 cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat
     4021 cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat
     4081 accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat
     4141 ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa
     4201 ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc
     4261 gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa
     4321 ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaggg gatcccccgg
     4381 gctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg ttctccggcc
     4441 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4501 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4561 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     4621 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4681 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4741 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4801 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4861 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     4921 ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg
     4981 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5041 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5101 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5161 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5221 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5281 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5341 atctcatgct ggagttcttc gcccaccccc cggatctaag ctctagataa gtaatgatca
     5401 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5461 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5521 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5581 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggg
     5641 ggtaccgcgt cgagcgccag tgtgatggat atctgcagaa ttcggcttga agttcctatt
     5701 ctctagaaag tataggaact tcagatctat gattcccttt gtcaacagca atggatcact
     5761 gaaaatggtt caatgatcac attaagtggt attcaatatt ttcatgaaat gggaattgac
     5821 gttccttcca aacattcacg taaaatctgt tgtgcgtgtt tagattggag tgaacgccgt
     5881 ttccatttag gtgggtacgt tggagccgca ttattttcgc tttatgaatc taaagggtgg
     5941 ttaactcgac atcttggtta ccgtgaagtt accatcacgg aaaaaggtta tgctgctttt
     6001 aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga
     6061 ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg
     6121 gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat
     6181 cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct
     6241 ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt
     6301 tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac
     6361 atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc
     6421 aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct
     6481 tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac
     6541 gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt
     6601 tacttttatc taatctagac atcattaatt cctaattttt gttgacactc tatcattgat
     6661 agagttattt taccactccc tatcagtgat agagaaaagt gaaatgaata gttcgacaaa
     6721 gatcgcattg gtaattacgt tactcgatgc catggggatt ggccttatca tgccagtctt
     6781 gccaacgtta ttacgtgaat ttattgcttc ggaagatatc gctaaccact ttggcgtatt
     6841 gcttgcactt tatgcgttaa tgcaggttat ctttgctcct tggcttggaa aaatgtctga
     6901 ccgatttggt cggcgcccag tgctgttgtt gtcattaata ggcgcatcgc tggattactt
     6961 attgctggct ttttcaagtg cgctttggat gctgtattta ggccgtttgc tttcagggat
     7021 cacaggagct actggggctg tcgcggcatc ggtcattgcc gataccacct cagcttctca
     7081 acgcgtgaag tggttcggtt ggttaggggc aagttttggg cttggtttaa tagcggggcc
     7141 tattattggt ggttttgcag gagagatttc accgcatagt ccctttttta tcgctgcgtt
     7201 gctaaatatt gtcactttcc ttgtggttat gttttggttc cgtgaaacca aaaatacacg
     7261 tgataataca gataccgaag taggggttga gacgcaatcg aattcggtat acatcacttt
     7321 atttaaaacg atgcccattt tgttgattat ttatttttca gcgcaattga taggccaaat
     7381 tcccgcaacg gtgtgggtgc tatttaccga aaatcgtttt ggatggaata gcatgatggt
     7441 tggcttttca ttagcgggtc ttggtctttt acactcagta ttccaagcct ttgtggcagg
     7501 aagaatagcc actaaatggg gcgaaaaaac ggcagtactg ctcggattta ttgcagatag
     7561 tagtgcattt gcctttttag cgtttatatc tgaaggttgg ttagttttcc ctgttttaat
     7621 tttattggct ggtggtggga tcgctttacc tgcattacag ggagtgatgt ctatccaaac
     7681 aaagagtcat cagcaaggtg ctttacaggg attattggtg agccttacca atgcaaccgg
     7741 tgttattggc ccattactgt ttgctgttat ttataatcat tcactaccaa tttgggatgg
     7801 ctggatttgg attattggtt tagcgtttta ctgtattatt atcctgctat cgatgacctt
     7861 catgttaacc cctcaagctc aggggagtaa acaggagaca agtgcttagt tatttcgtca
     7921 ccaaatgatg ttattccgcg aaatataatg accctcttga taacccaaga gggcattttt
     7981 tacgataaag aagatttagc ttcaaataaa acctatctat tttatttatc tttcaagctc
     8041 aataaaaagc cgcggtaaat agcaataaat tggccttttt tatcggcaag ctcttttagg
     8101 tttttcgcat gtattgcgat atgcataaac cagccattga gtaagttttt aagcacatca
     8161 tcatcataag cttgaagttc ctattctcta gaaagtatag gaacttcaag ccgaattcca
     8221 gcacactggc ggccgttact agttctagag cggccgccac cgcggtggag ctccagcttt
     8281 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
     8341 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8401 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     9181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10441 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
