LOCUS       sequence-39871-e         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, 
            Washburn MP, Dynlacht B, Pagano M
  TITLE     SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity 
            through CP110 degradation.
  JOURNAL   Nature. 2010 Jul 1;466(7302):138-42.
  PUBMED    20596027
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-07-1"; volume: "466(7302)"; pages: "138-42"
COMMENT     Sequence Label: pcDNA3-FLAG-CyclinF-Delta-F
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             112..135
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagactctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttaccat ggactacaag
      121 gacgacgatg acaaggtacc aggatccatg gggagcggcg gcgtggtcca ctgtaggtgt
      181 gccaagtgtt tctgttatcc tacaaagcga agaataagga ggaggccccg aaacctgacc
      241 atcttgagtg ccgccgaaga tgtgctcttt cacatcctga aatggctttc tgtagaggac
      301 atcctggccg tccgagctgt acactcccag ctgaaggacc tggtggacaa ccacgccagt
      361 gtgtgggcat gtgccagctt ccaggagctg tggccgtctc cagggaacct gaagctcttt
      421 gaaagggctg ctgaaaaggg gaatttcgaa gctgctgtga agctgggcat agcctacctc
      481 tacaatgaag gcctgtctgt gtctgatgag gcccgcgcag aagtgaatgg cctgaaggcc
      541 tctcgcttct tcagtctcgc tgagcggctg aatgtgggtg ccgcaccttt catctggctc
      601 ttcatccgcc ctccgtggtc ggtgagcgga agctgctgc
//
