LOCUS       sequence-39874-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   D'Angiolella V, Donato V, Vijayakumar S, Saraf A, Florens L, 
            Washburn MP, Dynlacht B, Pagano M
  TITLE     SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity 
            through CP110 degradation.
  JOURNAL   Nature. 2010 Jul 1;466(7302):138-42.
  PUBMED    20596027
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2010-07-1"; volume: "466(7302)"; pages: "138-42"
COMMENT     Sequence Label: pBABE-2XFLAG,2XHA-CyclinF-puro
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             43..69
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             43..69
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 accatggact acaaggacga cgatgacaag ctcgatggag gataccccta cgacgtgccc
       61 gactacgccg aaggaagatc catggggagc ggcggcgtgg tccactgtag gtgtgccaag
      121 tgtttctgtt atcctacaaa gcgaagaata aggaggaggc cccgaaacct gaccatcttg
      181 agtctccccg aagatgtgct ctttcacatc ctgaaatggc tttctgtaga ggacatcctg
      241 gccgtccgag ctgtacactc ccagctgaag gacctggtgg acaaccacgc cagtgtgtgg
      301 gcatgtgcca gcttccagga gctgtggccg tctccaggga acctgaagct ctttgaaagg
      361 gctgctgaaa aggggaattt cgaagctgct gtgaagctgg gcatagccta cctctacaat
      421 gaaggcctgt ctgtgtctga tgaggcccgc gcagaagtga atggcctgaa ggcctctcgc
      481 ttcttcagtc tcgctgagcg gctgaatgtg ggtgccgcac ctttcatctg gctcttcatc
      541 cgccctccgt ggtcggtgag cggaagctgc tgcaaggccg tggttcacga gagcctcagg
      601 gcagagtgcc agctgcagag gactcacaaa gcatccatat tgcactgctt gggcagagtg
      661 ctgagtctgt tc
//
