LOCUS       sequence-39875-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, 
            Niederweis M
  TITLE     Energy transfer between fluorescent proteins using a co-expression 
            system in Mycobacterium smegmatis.
  JOURNAL   Gene. 2001 Oct 31;278(1-2):115-24.
  PUBMED    11707328
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-10-31"; volume: "278(1-2)"; pages: "115-24"
COMMENT     Sequence Label: pMycVec1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..118)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      247..835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     736..755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agctaaagct tgatatcgaa ttcctgcagc ccgggggatc catttaaatc agctgttaat
       61 taatctagag tttaaacagt attaaacgca aaaaggccat ccgtcaggat ggccttctgc
      121 ttagctaatc aactagaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      361 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
      421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      781 ctgacttgag cgtcgatttt tgtgatgctc gtcanggggg cggagcctat ggaaaaacgc
      841 cagcaacgcg gcctttttac ggttcctggc
//
