LOCUS       sequence-39877-e         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Flavell SW, Cowan CW, Kim TK, Greer PL, Lin Y, Paradis S, Griffith 
            EC, Hu LS, Chen C, Greenberg ME
  TITLE     Activity-dependent regulation of MEF2 transcription factors 
            suppresses excitatory synapse number.
  JOURNAL   Science. 2006 Feb 17;311(5763):1008-12.
  PUBMED    16484497
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2006-02-17"; volume: "311(5763)"; pages: "1008-12"
COMMENT     Sequence Label: Mef2A-shRNA
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4..20
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        40..254
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     175..194
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(327..343)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(328..344)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(372..390)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(411..427)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(411..427)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(424..446)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    435..451
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(459..465,466..483,484..489))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    504..525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(642..659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgctctagaa ctagtggatc ccccgggctg caggaattcg aacgctgacg tcatcaaccc
       61 gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg cgtgcgccct
      121 ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg catgtcgcta
      181 tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat cttataagtt
      241 ctgtatgaga ccacagatcc ccgttatctc agggttcaaa tttcaagaga atttgaaccc
      301 tgagataact ttttggaaaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
      361 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
      421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      481 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact c
//
