LOCUS       sequence-39878-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Stephan J, Stemmer V, Niederweis M
  TITLE     Consecutive gene deletions in Mycobacterium smegmatis using the 
            yeast FLP recombinase.
  JOURNAL   Gene. 2004 Dec 8;343(1):181-90.
  PUBMED    15563844
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2004-12-8"; volume: "343(1)"; pages: "181-90"
COMMENT     Sequence Label: pMN252
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..74
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcgataagct cccgtttaaa ctcgcgaacc aggtactagt gaagttccta ttctctagaa
       61 agtataggaa cttcgttaac gaaatcaatc taaagtatat atgagtaaac ttggtctgac
      121 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
      181 atagttgcct gactccccga cgtggccgac cagcccgtca tcgtcaacgc ctgatccgcg
      241 gtgcggacag gccgtgtcgc gaccggccgt gcggaattaa gccggcccgt accctgtgaa
      301 tagaggtccg ctgtgacaca agaatccctg ttacttctcg accgtattga ttcggatgat
      361 tcctacgcga gcctgcggaa cgaccaggaa ttctgggagc cgctggcccg ccgagccctg
      421 gaggagctcg ggctgccggt gccgccggtg ctgcgggtgc ccggcgagag caccaacccc
      481 gtactggtcg gcgagcccgg cccggtgatc aagctgttcg gcgagcactg gtgcggtccg
      541 gagagcctcg cgtcggagtc ggaggcgtac gcggtcctgg cggacgcccc ggtgccggtg
      601 ccccgcctcc tcggccgcgg cgagctgcgg cccggcaccg gagcctggcc gtggccctac
      661 ctggtgatga gccggatgac cggcaccacc tggcggtccg cgatggacgg cacgaccgac
      721 cggaacgcgc tgctcgccct ggcccgcgaa ctcggccggg tgctcggccg gctgcacagg
      781 gtgccgctga ccgggaacac cgtgctcacc ccccattccg aggtcttccc ggaactgctg
      841 cgggaacgcc gcgcggcgac cgtcgaggac caccgcgggt ggggctacct ctcgccccgg
      901 c
//
