LOCUS       sequence-39880-e         474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Flavell SW, Cowan CW, Kim TK, Greer PL, Lin Y, Paradis S, Griffith 
            EC, Hu LS, Chen C, Greenberg ME
  TITLE     Activity-dependent regulation of MEF2 transcription factors 
            suppresses excitatory synapse number.
  JOURNAL   Science. 2006 Feb 17;311(5763):1008-12.
  PUBMED    16484497
REFERENCE   2  (bases 1 to 474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2006-02-17"; volume: "311(5763)"; pages: "1008-12"
COMMENT     Sequence Label: Mef2D-shRNA
FEATURES             Location/Qualifiers
     source          1..474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..253
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     174..193
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(326..342)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..343)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(371..389)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(410..426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(410..426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(423..445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    434..450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cgctctagac tagtggatcc cccgggctgc aggaattcga acgctgacgt catcaacccg
       61 ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg
      121 gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat
      181 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
      241 tgtatgagac cacagatccc cgtagctctc tggtcactcc ttcaagagag gagtgaccag
      301 agagctactt tttggaaaag cttatcgata ccgtcgacct cgaggggggg cccggtacca
      361 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
      421 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaa
//
