LOCUS       sequence-39886-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Cowley SC, Av-Gay Y
  TITLE     Monitoring promoter activity and protein localization in 
            Mycobacterium spp. using green fluorescent protein.
  JOURNAL   Gene. 2001 Feb 21;264(2):225-31.
  PUBMED    11250077
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2001-02-21"; volume: "264(2)"; pages: "225-31"
COMMENT     Sequence Label: pSC301
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..47
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(155..183)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cacccgcgcg aaaagaaaca tcggacgatc atttcatcga aggaaggaat gccgtgggat
       61 cccccgggct gcaggaattc gatatcaagc taattctgat taactttata aggaggaaaa
      121 acatatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      301 acttgtcact actttgactt atggtgttca atgcttttca agatacccag atcatatgaa
      361 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      421 tttcaaagat gacgggaact ataagacacg tgctgaagtc aagtttgaag gtgatacact
      481 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      541 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      601 tggaatcaaa gttaacttca aaatt
//
