LOCUS       sequence-39888-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Stemmann O, Zou H, Gerber SA, Gygi SP, Kirschner MW
  TITLE     Dual inhibition of sister chromatid separation at metaphase.
  JOURNAL   Cell. 2001 Dec 14;107(6):715-26.
  PUBMED    11747808
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2001-12-14"; volume: "107(6)"; pages: "715-26"
COMMENT     Sequence Label: pCS2-hSecurin
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             128..301
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             302..475
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             495..506
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             518..538
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             554..574
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             626..646
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             662..682
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tccgccatgg cgcaacacga
      121 tgaagccgta gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca
      181 tttacctaac ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc
      241 aagccaaagc gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa
      301 agtagacaac aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc
      361 taacttaaac gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca
      421 aagcgctaac cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga
      481 cgcgaattcg ctcgatcgag ggccgcccgg cagcagcgag aacctgtact tccagggcgc
      541 catccccacc accgagaacc tgtacttcca gagcggcacc gtggatgccg gcgccgatgc
      601 cggcaagccc cggcccggca gcagcgagaa cctgtacttc cagggcgcca tccccaccac
      661 cgagaacctg tacttccaga gcggcaccgt ggatgccggc gccgatgccg gcaagccccg
      721 gccgatgagg agcttcaaaa gaggccggcc gatggctact ctgatctatg ttgataagga
      781 aaatggagaa ccaggcaccc gtgtggttgc taaggatggg ctgaagctgg ggtctggacc
      841 ttcaatcaaa gccttagatg ggagatctca agtttcaaca ccacgttttg gcaaaacgtt
      901 cgatgcccca ccagccttac ctaaagctac tagaaaggct ttgggaactg tcaacagagc
      961 tacag
//
