LOCUS       sequence-399016-       11920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11920)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0345
FEATURES             Location/Qualifiers
     source          1..11920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7777..8365
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7830..7850)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8248..8259)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8437..8670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8748..8869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8785..8804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8839..8858
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8909..9044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9064..9083)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9065..9083)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9093..9110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9093..9109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9102..9124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9251..9706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9338..9357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9548..9569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9732..9836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9837..9905,9906..10697)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10055..10074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10868..11456
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11357..11376
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11610..11627
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11744..11765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11780..11785,11786..11803,11804..11810)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11818..11834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11823..11845
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11842..11858
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11842..11858
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11877..11897
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11879..11897
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gtggacaccg aggagaagcg
     4141 gcatcgcacg cggtccaaag gggttcgagt tcccgtggaa ccagccatac aagagctgtt
     4201 cagctgtccc acccctggct gtgacggcag tggtcatgtc agtggcaaat atgcaagaca
     4261 cagaagtgta tatggttgtc ccttggcgaa aaaaagaaaa acacaagata aacagcccca
     4321 ggaacctgct cctaaacgaa agccatttgc cgtgaaagca gacagctcct cagtggatga
     4381 gtgtgacgac agtgatggga ctgaggacat ggatgagaag gaggaggatg agggggagga
     4441 gtactccgag gacaatgatg agccagggga tgaggacgag gaggacgagg agggggaccg
     4501 ggaggaggag gaggagatcg aggaggagga tgaggacgat gacgaggatg gagaagatgt
     4561 ggaggatgaa gaagaggaag aggaggagga ggaggaggag gaagaggaag aagaaaacga
     4621 agaccatcaa atgaattgtc acaatactcg aataatgcaa gacacagaaa aggatgataa
     4681 caataatgac gaatatgaca attacgatga actggtggcc aagtcattgt taaacctcgg
     4741 caaaatcgct gaggatgcag cctaccgggc caggactgag tcagaaatga acagcaatac
     4801 ctccaatagt ctggaagacg atagtgacaa aaacgaaaac ctgggtcgga aaagtgagtt
     4861 gagtttagac ttagacagtg atgttgttag agaaacagtg gactccctta aactattagc
     4921 ccaaggacac ggtgttgtgc tctcagaaaa catgaatgac agaaattatg cagacagcat
     4981 gtcgcagcaa gacagtagaa atatgaatta cgtcatgttg gggaagccca tgaacaacgg
     5041 actcatggaa aagatggtgg aggagagcga tgaggaggtg tgtctgagca gtctggagtg
     5101 tttgaggaat cagtgcttcg acctggccag gaagctcagt gagaccaacc cgcaggagag
     5161 gaatccgcag cagaacatga acatccgtca gcatgtccgg ccagaagagg acttccccgg
     5221 aaggacgccg gacagaaact actcggacat gctgaacctc atgcggctgg aggagcagtt
     5281 gagcccccgg tcgagagtgt ttgccagctg tgcgaaggag gatgggtgtc atgagcggga
     5341 cgacgatacc acctctgtga actcggacag gtctgaagag gtgttcgaca tgaccaaggg
     5401 gaacctgacc ctgctggaga aagccatcgc tttggaaacg gaaagagcaa aggccatgag
     5461 ggagaagatg gccatggaag ctgggaggag ggacaatatg aggtcatatg aggaccagtc
     5521 tccgagacaa cttcccgggg aggacagaaa gcctaaatcc agtgacagcc atgtcaaaaa
     5581 gccatactat gatccctcaa gaacagaaaa gaaagagagc aagtgtccaa cccccgggtg
     5641 tgatggaacc ggccacgtaa ctgggctgta cccacatcac cgcagcctgt ccggatgccc
     5701 gcacaaagat agggtccctc cagaaatcct tgccatgcat gaaagtgtcc tcaagtgccc
     5761 cactccgggc tgcacggggc gcgggcatgt caacagcaac aggaactccc accgaagcct
     5821 ctccggatgc ccgatcgctg cagcagagaa actggccaag gcacaggaaa agcaccagag
     5881 ctgcgacgtg tccaagtcca gccaggcctc ggaccgcgtg ctcaggccaa tgtgctttgt
     5941 gaagcagctg gagattcctc agtatggcta cagaaacaat gtccccacaa ctacgccgcg
     6001 ttccaacctg gccaaggagc tcgagaaata ttccaagacc tcgtttgaat acaacagtta
     6061 cgacaaccat acttatggca agcgagccat agctcccaag gtgcaaacca gggatatatc
     6121 ccccaaagga tatgatgatg cgaagcggta ctgcaaggac cccagcccca gcagcagcag
     6181 caccagcagc tacgcgccca gcagcagcag caacctgagc tgcggcgggg gcagcagcgc
     6241 cagcagcacg tgcagcaaga gcagcttcga ctacacgcac gacatggagg cggcccacat
     6301 ggcggccacc gccatcctca acctgtccac gcgctgccgc gagatgccgc agaacctgag
     6361 caccaagccg caggacctgt gcgccacgcg gaaccctgac atggaggtgg atgagaacgg
     6421 gaccctggac ctcagcatga acaagcagag gccgcgggac agctgctgcc ccatcctgac
     6481 ccctctggag cccatgtccc cccagcagca ggcagtgatg aacaaccggt gtttccagct
     6541 gggcgagggc gactgctggg acttgcccgt agactacacc aaaatgaaac cccggaggat
     6601 agacgaggac gagtccaaag acattactcc agaagacttg gacccattcc aggaggctct
     6661 agaagaaaga cggtatcccg gggaggtgac catcccaagt cccaaaccca agtaccctca
     6721 gtgcaaggag agcaaaaagg acttaataac tctgtctggc tgccccctgg cggacaaaag
     6781 cattcgaagt atgctggcca ccagctccca agaactcaag tgccccacgc ctggctgtga
     6841 tggttctgga catatcaccg gcaattatgc ttctcatcgg agcctttcag gttgcccaag
     6901 agcaaagaaa agtggtatca ggatagcaca gagcaaagaa gataaagaag atcaagaacc
     6961 catcaggtgt ccggtccccg ggtgcgacgg ccagggccac atcactggga agtacgcgtc
     7021 ccatcgcagc gcctccgggt gccccttggc ggccaagagg cagaaagacg ggtacctgaa
     7081 tggctcccag ttctcctgga agtcggtcaa gacggaaggc atgtcctgcc ccacgccagg
     7141 atgcgacggc tcaggccacg tcagcggcag cttcctcaca caccgcagct tgtcaggatg
     7201 cccgagagcc acgtcagcga tgaagaaggc aaagctttct ggagagcaga tgctgaccat
     7261 caaacagcgg gccagcaacg gtatagaaaa tgatgaagaa atcaaacagt tagatgaaga
     7321 aatcaaggag ctaaatgaat ccaattccca gatggaagcc gatatgatta aactcagaac
     7381 tcagattacc acgatggaga gcaacctgaa gaccatcgaa gaggagaaca aagtgattga
     7441 gcagcagaac gagtctctcc tccacgagct ggcgaacctg agccagtctc tgatccacag
     7501 cctggctaac atccagctgc cgcacatgga tccaatcaat gaacaaaatt ttgatgctta
     7561 cgtgactact ttgacggaaa tgtatacaaa tcaagatcgt tatcagagtc cagaaaataa
     7621 agccctactg gaaaatataa agcaggctgt gagaggaatt caggtctagt aatgaactag
     7681 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatgc
     7741 tctgcttgta cctagtgcgt tacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     7801 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7861 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7921 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7981 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     8041 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     8101 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     8161 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     8221 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     8281 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     8341 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     8401 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     8461 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     8521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8641 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     8701 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8881 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     9001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     9061 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     9121 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9181 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     9241 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9301 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9361 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9421 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9481 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9541 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9601 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9661 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9721 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9781 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9841 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9901 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9961 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
    10021 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
    10081 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
    10141 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
    10201 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
    10261 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
    10321 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
    10381 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
    10441 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
    10501 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10561 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10621 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10681 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10741 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10801 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10861 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10921 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10981 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    11041 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    11101 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    11161 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    11221 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    11281 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    11341 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    11401 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    11461 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    11521 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11581 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11641 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    11701 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11761 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11821 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    11881 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
