LOCUS       sequence-399021-       17359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17359)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 17359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0661
FEATURES             Location/Qualifiers
     source          1..17359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4456..4465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    13216..13804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(13269..13289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13687..13698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13876..14109
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    14187..14308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14224..14243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14278..14297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      14348..14483
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(14503..14522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14504..14522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14532..14549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14532..14548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14541..14563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14690..15145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14777..14796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14987..15008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        15171..15275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15276..15344,15345..16136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15494..15513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      16307..16895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16796..16815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17049..17066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17183..17204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17219..17224,17225..17242,17243..17249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17257..17273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17262..17284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17281..17297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17281..17297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     17316..17336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17318..17336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggca gatccaggaa tgatgagtct
     4141 ttttggcgag gatgggaata ttttcagtga aggtcttgaa ggcctcggag aatgtggtta
     4201 cccggaaaat ccagtaaatc ctatgggtca gcaaatgcca atagaccaag gctttgcctc
     4261 tttacagcca tcccttcatc atccttcaac taatcaaaat caaacaaagc tgacacattt
     4321 tgatcactat aatcagtatg aacaacaaaa gatgcatctg atggatcagc cgaacagaat
     4381 gatgagcaac acccctggga acggactcgc gtctccgcac tcgcagtatc acacccctcc
     4441 cgttcctcag gtgccccatg gtggcagtgg tggcggtcag atgggtgtct accctggcat
     4501 gcagaatgag aggcatgggc aatcctttgt ggacagcagc tccatgtggg gccccagggc
     4561 tgttcaggta ccagaccaga tacgagcccc ctaccagcag cagcagccac agccgcagcc
     4621 accgcagccg gctccgtcgg ggccccctgc acagggccac cctcagcaca tgcagcagat
     4681 gggcagctat atggcacgtg gggatttttc catgcagcag catggtcagc cacagcagag
     4741 gatgagccag ttttcccaag gccaagaggg cctcaatcag ggaaatcctt ttattgccac
     4801 ctcaggacct ggccacttgt cccacgtgcc ccagcagagt cccagcatgg caccttcctt
     4861 gcgtcactcg gtgcagcagt tccatcacca cccctctact gctctccatg gagaatccgt
     4921 tgcccacagt cccagattct ccccgaatcc tccccaacaa ggggctgtta ggccgcaaac
     4981 ccttaacttt agttctcgga gccagacagt cccctctcct actataaaca actcagggca
     5041 gtattctcga tatccttaca gtaacctaaa tcagggatta gttaacaata cagggatgaa
     5101 tcaaaattta ggccttacaa ataatactcc aatgaatcag tccgtaccaa gataccccaa
     5161 tgctgtagga ttcccatcaa acagtggtca aggactaatg caccagcagc ccatccaccc
     5221 cagtggctca cttaaccaaa tgaacacaca aactatgcat ccttcacagc ctcagggaac
     5281 ttatgcctct ccacctccca tgtcacccat gaaagcaatg agtaatccag caggcactcc
     5341 tcctccacaa gtcaggccgg gaagtgctgg gataccaatg gaagttggca gttatccaaa
     5401 tatgccccat cctcagccat ctcaccagcc ccctggtgcc atgggaatcg gacagaggaa
     5461 tatgggcccc agaaacatgc agcagtctcg tccatttata ggcatgtcct cggcaccaag
     5521 ggaattgact gggcacatga ggccaaatgg ttgtcctggt gttggccttg gagacccaca
     5581 agcaatccag gaacgactga tacctggcca acaacatcct ggtcaacagc catcttttca
     5641 gcagttgcca acctgtcctc cactgcagcc tcacccgggc ttgcaccacc agtcttcacc
     5701 tccacaccct catcaccagc cttgggcaca gctccaccca tcaccccaga acaccccgca
     5761 gaaagtgcct gtgcatcagc attccccgtc ggagcccttt ctagagaaac cagtgccgga
     5821 tatgactcag gttagtggac cgaatgctca gctagtgaag agtgatgatt acctgccatc
     5881 aatagaacag cagccacaac aaaagaagaa gaaaaagaaa aacaaccaca ttgtagcaga
     5941 ggatcccagt aaaggttttg gtaaagatga cttccctggt ggggtagata accaagaact
     6001 aaataggaac tcactggatg ggtcccaaga agaaaaaaag aaaaagaaaa ggtcaaaggc
     6061 aaaaaaagac ccgaaggaac cgaaagaacc caaggagaaa aaagagccca aggaacccaa
     6121 gaccccgaaa gcccctaaga ttcccaaaga gccaaaggaa aagaaagcaa aaactgccac
     6181 gccaaaaccc aaatccagca aaaagtcaag taataagaaa cctgactcag aagcaagtgc
     6241 tttgaagaaa aaggtcaaca agggaaaaac agaaggttct gaaaattcag acttagacaa
     6301 aacaccccca ccatctcctc ctcctgaaga agatgaggac ccaggtgttc agaagagacg
     6361 gtccagcaga caggtgaaga gaaagcgcta cactgaagac ctggagttca agatttctga
     6421 tgaggaggca gatgatgcag atgctgctgg gagggattcc ccctccaaca cctcccagtc
     6481 agaacagcag gaatctgttg atgcagaagg cccagtggta gaaaaaatta tgagcagtcg
     6541 ttcagtaaaa aagcagaagg aatctggaga ggaggtagaa attgaggaat tctatgtgaa
     6601 atacaaaaac ttctcttatc ttcattgtca gtgggcatct atagaagatc tggaaaaaga
     6661 taagagaatt cagcaaaaaa ttaaacgatt taaggcaaag cagggccaga acaagttcct
     6721 ttcagagatt gaggatgagc tttttaatcc agattatgtg gaggttgacc ggataatgga
     6781 ctttgcacgt agcacagatg accggggaga gcctgtgact cactatctgg tgaagtggtg
     6841 ttcacttcct tatgaagaca gcacgtggga gcggaggcag gacatagatc aagcaaagat
     6901 cgaggagttt gagaaactaa tgtccaggga gccggaaaca gagcgtgtgg agcgacctcc
     6961 tgctgatgat tggaagaaat cggagagttc cagggagtat aaaaacaata acaaactcag
     7021 ggaataccag ttggagggag taaactggct acttttcaat tggtacaaca tgcgaaactg
     7081 cattttagca gatgaaatgg gtttgggaaa aactatccag tccattacat ttctctatga
     7141 gatatatttg aaaggaatcc atggcccttt tttagtaatt gccccattgt ccacaatccc
     7201 caactgggaa agggaattcc gaacctggac agagttgaac gtggttgtgt atcatgggag
     7261 tcaagctagt cgtcggacca ttcagttgta tgaaatgtac ttcaaagatc cccagggtcg
     7321 agtgataaag gggtcctata agtttcatgc catcatcact acatttgaga tgattttgac
     7381 tgattgtcct gagctgcgga atattccatg gcgctgtgta gtcattgatg aagcccacag
     7441 gctgaagaac aggaactgca agctgttgga gggactcaag atgatggact tggaacacaa
     7501 agtgctgctg acgggaaccc cactccagaa cactgtggaa gaactcttca gcttgcttca
     7561 tttcttggaa ccaagtcgct tcccttcaga aaccacattt atgcaagaat ttggtgatct
     7621 aaaaacagaa gagcaggtgc aaaaacttca agctattcta aagccaatga tgttgagacg
     7681 tctcaaagag gatgtagaaa agaacttggc ccccaaagaa gaaactatta ttgaagttga
     7741 gctaacaaac attcagaaga aatattaccg agccatcctt gagaagaatt tcacatttct
     7801 ttccaaaggc ggtggtcaag ctaacgtacc taacctatta aacactatga tggaattgcg
     7861 gaagtgctgc aatcatccgt accttatcaa tggtgctgaa gagaaaattt tggaagagtt
     7921 taaagaaaca cacaatgcag agtctccaga ttttcagctc caggcaatga tccaggctgc
     7981 tggcaagcta gtgctgattg acaagctgct gccaaaactg aaggctggtg gccacagggt
     8041 gcttatcttt tcccagatgg tgcgctgctt ggacatactg gaagactacc tcattcaaag
     8101 acggtaccca tatgaaagga tcgacggccg agtaagaggc aacctccgcc aggcagctat
     8161 cgacagattc tccaaacctg attctgatag gtttgttttc ctcctgtgta caagggcagg
     8221 aggtttaggc attaacctca ctgctgctga tacctgcatc atctttgatt cagactggaa
     8281 tccccaaaat gacctccagg ctcaggctag atgtcataga ataggacaga gcaaatctgt
     8341 gaaaatctac aggctgatta caagaaattc ctatgaaagg gaaatgttcg acaaggctag
     8401 tttgaaactg ggcctggata aagctgtgct acagtctatg agtggaagag aaaatgctac
     8461 caatggggta caacagcttt ccaagaaaga aatagaggat cttctacgaa aaggggccta
     8521 tggtgcactc atggatgagg aggatgaagg gtctaaattc tgtgaagaag atattgatca
     8581 gatcctccta cgtcgaaccc acaccattac cattgagtca gaagggaaag gttccacatt
     8641 tgctaaggcc agttttgttg catctggaaa taggacagat atttccttgg atgatccaaa
     8701 tttctggcaa aagtgggcta agaaggctga attggatatt gatgccttaa atgggaggaa
     8761 caacctggtt attgatactc caagagtgag aaagcagacc aggctctaca gtgcagtgaa
     8821 ggaagatgag ctgatggagt tctcagactt ggaaagtgat tctgaagaaa agccctgtgc
     8881 aaagccacgg cgtccccagg ataagtcaca gggctatgca aggagtgaat gtttcagggt
     8941 ggagaagaat ctgcttgtct atggttgggg acggtggaca gacattcttt cccacggacg
     9001 ctataaacgc caactcactg agcaagatgt agaaaccatc tgcagaacca tcctggtgta
     9061 ctgtcttaat cattacaaag gggatgagaa tatcaaaagc ttcatctggg atctgatcac
     9121 acccacagcg gatggccaga ctcgagcctt ggtcaaccat tccggtttgt cagctcctgt
     9181 gccaagggga aggaagggaa agaaggtgaa agcccagagc acacagccgg tggtgcagga
     9241 tgccgactgg ctggccagct gcaacccaga tgccctgttc caggaggaca gctacaagaa
     9301 acacctgaag catcactgta acaaggtcct gctgcgtgtc cgcatgctgt actacctaag
     9361 acaagaagtg ataggagacc aggcggataa gatcttagag ggtgctgact caagtgaagc
     9421 cgatgtgtgg atccctgaac ctttccatgc tgaagttcct gcagattggt gggataagga
     9481 agcagacaaa tccctcttaa ttggagtgtt caaacatggc tatgagaagt acaactccat
     9541 gcgagctgac cccgcgctgt gctttctgga acgagtcggt atgcctgatg ccaaggccat
     9601 agctgccgag caaagaggaa cagacatgct agcagatggt ggtgacgggg gagaatttga
     9661 tagagaagat gaagacccag aatataaacc aaccagaaca ccgttcaaag atgaaataga
     9721 tgaatttgca aattctcctt cagaggataa ggaagaatcc atggaaatac atgccacagg
     9781 caagcacagt gagagtaatg ctgagttagg ccaactttac tggcctaaca cttcaaccct
     9841 gactacacgt ctgcgccggc tcattactgc ctatcagcgc agctataaaa ggcaacagat
     9901 gaggcaagag gccctaatga agactgaccg gcgcagacgg cggcctcgag aggaagtgag
     9961 agctctggaa gcggaaaggg aagctattat atctgagaag cggcaaaagt ggacaagaag
    10021 agaagaggct gatttttacc gtgtggtatc cacctttggg gttatttttg accctgtgaa
    10081 acagcaattt gactggaacc aatttagagc ctttgccagg cttgacaaaa aatctgatga
    10141 gagtttggag aaatacttca gttgttttgt ggccatgtgt aggcgagtat gtcgaatgcc
    10201 cgtcaagcca gatgatgaac cgcccgacct ctcctccata attgagccga tcacagagga
    10261 gcgagcctct cgaactctgt accgcattga gctgctacgg aagatccgcg agcaggttct
    10321 ccatcacccc cagctgggag agaggcttaa gctctgccag ccaagcttgg atctgccaga
    10381 gtggtgggag tgtggacggc atgaccgaga cttgctggtt ggtgctgcta aacacggggt
    10441 cagtcggacg gattatcaca tcctcaatga ccctgagtta tccttcttgg atgcacataa
    10501 aaactttgct caaaacagag gggcaggtaa tacatcttcc ttgaacccac tggcagttgg
    10561 atttgtccag actcctccag tcatctcatc tgctcatatt caagatgaga gggtactgga
    10621 acaagccgaa ggcaaagtgg aggagcctga aaacccagct gccaaggaga aatgtgaggg
    10681 caaagaagag gaagaagaaa ccgatggcag cgggaaggag agcaagcagg aatgtgaggc
    10741 agaggccagc tctgtgaaaa atgaactgaa aggtgttgag gtcggcgcag acactgggtc
    10801 caaatctatt tcagagaaag gttccgaaga ggatgaagag gaaaagctgg aggatgacga
    10861 taagtcggaa gagtcttccc agcccgaagc aggagctgtc tctagaggga agaattttga
    10921 tgaagaaagc aatgcttcca tgagcactgc tagagatgaa acccgagatg gattctacat
    10981 ggaggacgga gatccttcag tagctcagct ccttcatgaa agaacatttg ccttctcgtt
    11041 ttggcctaag gatagagtaa tgataaaccg cttagacaac atctgtgaag cagtgttgaa
    11101 aggcaaatgg ccagtaaata ggcgccagat gtttgatttc caaggcctca tcccaggtta
    11161 cacacccacc acagtggaca gccccttgca gaagaggagc tttgctgagc tctccatggt
    11221 cggccaagcc agcattagtg ggagtgagga catcactacg tctcctcagt tgtcaaagga
    11281 agatgccctc aacctctctg tccctcgcca gcggaggagg aggaggagaa aaatcgaaat
    11341 tgaggccgaa agagctgcca agaggcgaaa tctcatggag atggttgccc agcttcgaga
    11401 gtctcaggtg gtctcagaaa atggacaaga aaaagttgta gatttatcaa aggcctcaag
    11461 agaggcaaca agctctacct caaatttttc atctctttct tcaaagttta tcttgcctaa
    11521 tgtctcaaca ccagtgtctg atgcctttaa gactcaaatg gaactgctcc aagcaggcct
    11581 ttcgcgcaca cccacaaggc atctccttaa tggctcccta gtggatggag agcctcccat
    11641 gaagaggagg cggggaagga ggaaaaatgt ggagggactt gatctgcttt tcatgagcca
    11701 caaacggacg tcattgagtg cagaggatgc tgaggtgacc aaagcttttg aagaagatat
    11761 agagacccca ccaacaagaa acattccttc tcccggacag ctggacccag acacacggat
    11821 ccctgttatc aatcttgaag atgggactag gctggtgggg gaagatgctc ctaaaaataa
    11881 ggatttagtt gaatggctga agctgcaccc tacttacact gttgatatgc caagttatgt
    11941 accaaagaat gcagatgtgc tgttttcctc atttcagaaa ccgaaacaga aacgacatag
    12001 atgtcgaaac cctaataaat tggatataaa cactttgaca ggagaagaaa gggtgcctgt
    12061 tgtcaataaa cgaaatggga agaagatggg tggagctatg gcgcctccaa tgaaggatct
    12121 acccaggtgg ctggaagaaa atcctgaatt tgcagttgct ccagactgga ctgatatagt
    12181 taagcagtct ggttttgttc ctgagtcgat gtttgaccgc cttctcactg ggcctgtagt
    12241 gcggggagag ggagcgagca gaagaggaag aaggcccaaa agtgagatcg ccagagcagc
    12301 cgcggccgcc gctgctgtgg cctccacgtc agggatcaac cctttgctgg tgaacagcct
    12361 gtttgctgga atggacctga cgagccttca gaatctccag aatctccagt cgctccagct
    12421 ggcaggcctc atgggcttcc ctccaggact ggcaacagct gccaccgccg gaggcgatgc
    12481 gaagaaccct gctgctgtgc tgcccctgat gctgccagga atggcgggcc tgcccaacgt
    12541 gtttggcttg ggcgggctgt tgaataaccc tctgtcagct gctactggaa acaccactac
    12601 tgcttctagt caaggagaac cggaagacag cacttcaaaa ggagaggaga aaggaaatga
    12661 gaatgaagac gagaacaaag actctgagaa aagcacagat gctgtttcgg ctgctgactc
    12721 tgcgaatgga tctgttggtg ctgctactgc cccggctgga ttgccctcaa acccgctagc
    12781 cttcaaccct ttcctcctgt ccacaatggc cccgggcctc ttctacccat ccatgtttct
    12841 acctccagga ctggggggat tgacgctgcc tgggttccca gcattggcag gacttcagaa
    12901 tgccgtgggc tccagcgaag aaaaggctgc tgacaaggct gagggaggac cctttaaaga
    12961 tggagagacc cttgaaggca gcgatgccga ggagagcctg gataagactg cagagtcctc
    13021 cctcttagaa gacgaaatag cacagggtga agagctagac tcacttgatg ggggggatga
    13081 aatagaaaac aatgaaaatg atgaatagta atgaactagt ccgtaacttg aaagtatttc
    13141 gatttcttgg ctttatatat cttgtggaaa ggacgaagac cctcctcaac acaatactgt
    13201 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
    13261 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
    13321 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
    13381 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
    13441 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
    13501 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
    13561 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
    13621 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
    13681 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
    13741 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
    13801 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    13861 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    13921 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    13981 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    14041 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    14101 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
    14161 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
    14221 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    14281 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
    14341 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    14401 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    14461 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
    14521 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    14581 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    14641 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
    14701 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
    14761 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    14821 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    14881 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    14941 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    15001 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    15061 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    15121 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
    15181 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    15241 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    15301 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    15361 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    15421 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    15481 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    15541 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    15601 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    15661 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    15721 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    15781 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    15841 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    15901 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    15961 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    16021 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    16081 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    16141 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    16201 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    16261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    16321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    16381 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    16441 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    16501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    16561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    16621 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    16681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    16741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    16801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    16861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    16921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    16981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    17041 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    17101 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    17161 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    17221 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    17281 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    17341 aaaagctgga gctgcaagc
//
